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Table 1 Identification of abundant proteins in Capitella Sp I during metamorphosis by MALDI-TOF/TOF.

From: Differential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. I

Spot No.

Acc No.a)

Protein name b)

MW(kDa)c)

Obs./Theo.

pIc)

Obs./Theo.

PM/SC (%)

Fold

Change

(Total protein)

Fold

Change

(mRNA)

17

163388/P68370

Alpha -tubulin (α-TUB)

40/50

5.6/5.5

35/11

-2.4

-1.1

18

156132/P11833

Beta-tubulin ((β-TUB)

43/45

5.7/5.3

20/6

-2.7

NA

19

157862/Q9PTY0

ATP synthase beta subunit

(ATP2)

46/44

5.0/5.3

33/9

+1.9

+ 2.2

20

158679/P68142

Actin cytoplasmic type 8 (ACT)

42/42

5.3/5.6

16/4

-1.1

+ 1.6*

21

158679/P68142

Actin cytoplasmic type 2 (ACT)

59/40

5.6/5.5

16/9

-1.1

+ 1.6*

22

158679/P68142

Beta-actin(ACT)

39/40

5.7/5.6

34/12

-1.1

+ 1.6*

23

158616/Q5R546

ATP synthase alpha subunit (ATP2)

59/56

8.5/7.0

38/23

+1.9

+ 2.2

24

163979/P48735

Isocitrate dehydrogenase-2 (ICH)

44/48

5.9/6.4

27/9

-1.5

NA

25

160805/P79818

Cytoplasmic beta actin (ACT)

40/40

5.6/6.0

25/7

-1.1

NA

26

226906/Q6GM17

Enolase-phosphatase E1 (EP1)

21/40

4.1/4.3

27/5

-1.5

NA

27

153159/Q01105

Template activating factor (TAF)

30/35

4.1/4.3

26/5

+1.5

+ 2.5

28

156731/P43689

Tropomyosin-2(TMII)

33/33

4.6/4.5

40/12

+1.8

NA

29

152326/P62258

Tyrosine 3-monooxygenase (TH)

29/32

4.7/4.7

25/8

-2.2

-1.1

30

158679/P68142

Actin cytoplasmic type 1 (ACT)

41/35

5.5/5.3

31/8

-1.1

+ 1.6*

31

221075

Conserved hypothetical protein (HP)

52/43

7.8/6.2

15/7

NA

NA

32

221075

Conserved hypothetical protein (HP)

52/40

7.8/6.5

31/16

NA

NA

33

209306/P55155

Vitellogenin (Vtg)

176/35

7.1/6.3

8/10

-8.0

-2.0*

34

209306/P55155

Vitellogenin (Vtg)

176/33

7.1/6.3

11/13

-8.0

-2.0*

35

180369/Q63716

Thioredoxin peroxidase (TPx)

26/28

6.7/6.2

41/8

+1.1

-1.4

36

175529/Q23280

hypothetical protein (HP)

23/29

4.3/4.3

11/3

NA

NA

37

209306/P05690

Vitellogenin (Vtg)

176/18

7.1/7.0

11/13

-3.0

-2.0*

38

198200/P05690

Vitellogenin (Vtg)

174/14

7.9/5.5

3/7

-2.0

-2.0*

39

159573/P07900

Heat shock protein-90 (HSP90)

95/45

4.8/5.0

7/18

-1.8

NA

  1. a) Accession numbers are from the JGI Capitella genome database and the Swiss-Prot database b) For positive identification, the score had to be over the significance threshold (p<0.05). c) Observed (Obs.) MW and pI values were estimated from 2-DE gels and Theoretical (Theo.) MW and pI values were derived from a database search by MASCOT. PM: number of peptides matching the protein sequence; SC: sequence coverage; *: Total mRNA expression levels; NA: Not determined.