Skip to main content

Table 2 Gene-specific primers used for real-time PCR

From: Differential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. I

Name of the gene Accession no. Primer sequence
Template activating factor 1 153159 FP: 5'-GGCCCAACCCCCTCCAGTAC-3'
RP: 5'-AGCCAGAATGGGCTGTGGTGC-3'
Actin, cytoplasmic 158679 FP: 5'-ACGAAGTTGCCGCTCTTGTCATC-3'
RP: 5'-GCCCATACCGACCATGACACCC-3'
ATP synthase beta subunit 157862 FP: 5'-CCGCCACTCCCAAGGGCAAA-3'
RP: 5'-CGCACTCCTGGCCACGGATC-3'
Thirodoxin peroxidase 180369 FP: 5'-TGCGCCAGGTGACCATCAACG-3'
RP: 5'-GCAGCACCTCATCGACCGACC-3'
Alpha tubulin 163388 FP: 5'-CACGTCCCCCGTGCCGTAA-3'
RP: 5'-CCAGTGCGGACCTCATCAACC-3'
Vitellogenin 209306 FP: 5'-GTCCGCGCAGCGCTCAAATG-3'
RP: 5'-GGAGCTGGCGGAACGAAGCA-3'
Tyrosine monooxygenase 152326 FP: 5'-CGGGCGCAGCCATCTTGATTG-3'
RP: 5'-ACGCGACGGACAGCAGGTTTC-3'
18s RNA
(Reference gene)
AF508118 FP: 5'-GGAAAACTCACCCGGCCCGG-3'
RP: 5'-CGACCCGCAGAACGGATCGG-3'