Software Architecture of MicroVigene™for High Throughput Protein Microarray Analysis. MicroVigene™ integrates protein array image analysis and statistical signal data analyses in a single automated process. The array images are analyzed in a batch process. Data analyses including background correction, statistics of replicates, dilution curve fitting, quality controls, normalization can be carried out automatically. The final outputs are the EC50 values from each serial dilution arranged in a spread sheet that can be further analyzed by other Bioinformatics tools.