Figure 8From: Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions Regional Background Correction Algorithm. This algorithm is to correct the original spot signal intensity (A) with local background (B). The real spot signals (C) can be extracted. Four spots within the black dashed square boxes in 2D view are shown in the 3D view windows below. This algorithm improves accuracy, consistency, and sensitivity for image processed using MicroVigeneâ„¢.Back to article page