Skip to main content
Figure 4 | Proteome Science

Figure 4

From: Differential proteome analysis of human embryonic kidney cell line (HEK-293) following mycophenolic acid treatment

Figure 4

Differential expression of Prdx1 and MLC2 by MPA treatment. (a) Selected areas in the silver stained gels showing differential expression of Prdx1 and MLC2. Delta 2D software was used for densitrometric analysis. The quantification of the level of expression (% volume) in MPA treated cells and control cells (DMSO) is illustrated as a bar chart with the mean and SD of four separate experiments (*p < 0.05). (b) Expression patterns of Prdx1 and MLC2 genes determined by real-time PCR. The relative expression of Prdx1 and MLC2 mRNA in the treated samples was determined as a fold change compared with control samples using the comparative threshold cycle (CT) method (2-ΔΔC T) as described in materials and methods part. Results shown are representative of four independent experiments. EF-2 was used to normalize the values. The boxes represent range in variation statistics and the lines across the boxes represent the medians and the whiskers extend to the highest and lowest values. Significance was calculated using the Mann-Whitney-U test (*p < 0.05) (c) Effect of MPA treatment on Prdx1 and MLC2 protein expression. Protein extracts from MPA and DMSO treated cells were Western blotted using specific antibodies against Prdx1 and MLC2. Densitometric analysis was done using Lab image version 2.71 software. β tubulin signal was used to control the equal protein load. The experiments were repeated four times and error bars represent ± SD (**p < 0.005).

Back to article page