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Table 1 Differentially regulated proteins by MPA in HEK-293 cells identified by mass spectrometry

From: Differential proteome analysis of human embryonic kidney cell line (HEK-293) following mycophenolic acid treatment

Spot No Acc Mt/Mo(kDa) Score pIt/pIo Pep Protein name FunctionBy KOGnitor NCBI Expression change (in folds)
6 Q01105 33.4/37.0 154 4.23/4.14 3 Protein SET Replication, recombination and repair 1.86*↓
9 Q07021 31.3/31.0 141 4.74/4.5 3 Complement component 1 Q subcomponent-binding protein, mitochondrial Defense mechanisms 1.58*↑
14 P38117 27.8/25.5 181 8.24/9.18 6 Electron transfer flavoprotein subunit beta Energy production and conversion 1.54*↑
15 P47985 29.6/25.0 112 8.51/7.081 6 Cytochrome b-c1 complex subunit Rieske, mitochondrial Energy production and conversion 3.71**↑
18 Q06830 22.0/21.0 64 8. 27/8.14 2 Peroxiredoxin-1 Posttranslational modification, protein turnover, chaperones 1.71**↑
22 P16949 17.2/15.8 56 5.76/6.32 3 Stathmin General function prediction only 1.50**↓
23 O95881 19.1/16.0 123 5.24/5.89 4 Thioredoxin domain-containing protein 12 Cytoskeleton 1.95*↑
24 O14950 19.7/16.0 195 4.71/5.32 4 Myosin regulatory light chain MRLC2 Cytoskeleton 3.41*↑
27 Q96A08 14.1/14.5 51 10.31/7.0 2 Histone H2B type 1-A Chromatin structure and dynamics 1.90*↓
28 P62807 13.8/14.2 250 10.31/6.51 9 Histone H2B type 1-C/E/F/G/I Chromatin structure and dynamics 1.58*↓
31 P25398 14.5/13.0 89 6.81/7.10 3 40 S ribosomal protein S12 Translation, ribosomal structure and biogenesis 2.44*↓
34 P07737 15.0/13.5 142 8.44/9.07 6 Profilin-1 Cytoskeleton 1.51**↑
  1. Acc: Accession number; Mt: theoretical molecular mass; Mo: observed molecular mass; pIt: theoretical isoelectric point; pIo: observed isoelectric point; pep: number of peptides sequenced for identification; Score: Peptide mass fingerprint probability score as defined by Mascot (www.matrixscience.com). Individual ions score > 42 indicate identity or extensive homology (p < 0.05); ↓: down-regulated; ↑ up-regulated; *p < 0.05, **p < 0.005. Molecular function determined from the online protein reference database KOGnitor NCBI (http://www.ncbi.nlm.nih.gov/COG/grace/kognitor.html).