Figure 3

Immunostaining of protein carbonyl moieties revealed significantly lower amount of oxidized proteins in cortex tissue than in striatum and midbrain in both PINK1-KO and wild type mice (ANOVA analysis followed by unpaired Student T-test, p < 0.05). A) A representative Oxyblot image of a 2DE gel immunostained against protein carbonyls (image from PINK1-KO, midbrain). B) Bar chart showing differential protein oxidation extents in striatum, midbrain and cortex tissues of PINK1-KO and control mice (mean ± SEM, n = 3. see Materials and Methods for detail). Hereby, the average signal intensity of protein oxidation in striatum of control mice was set as 100%. Experiments were repeated three times on exactly 100 μg of pooled protein extract of each brain region.