Electron microscopy revealed higher incidences of mitophagic vacuoles in cortex tissue of wild type mouse (designated as ** in figure. ANOVA test followed by unpaired student T-test, n = 30 for each brain region, p < 0.05). A) Insets of electron micrographs highlighting typical mitophagic vacuoles (arrow heads) that contain mitochondria and other cargos found in the cortex, striatum and substantia nigra. B) Bar chart showing the number of total mitochondria, mitophagic vacuoles and defective mitochondria in three mouse brain regions investigated (mean ± SEM). The cortex, striatum and substantia nigra (the most vulnerable area of midbrain in PD pathology) samples of three wild type mice were used for the electron microscopy investigation. Thirty micrographs were inspected for each tissue sample.