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Table 3 Twelve representative proteins from human SF identified by MS and separated by 2 - DE

From: Optimizing Human Synovial Fluid Preparation for Two-Dimensional Gel Electrophoresis

Spot namea Description Access Noa Mr(kDa)b pIb No. of Matchedc Seq Cov (%)d
A1BG Alpha-1B-glycoprotein P04217 54.79 5.56 15 30
ALBU Serum albumin P02768 71.32 5.92 27 54
APOA1 Apolipoprotein A-1 P02647 30.00 5.56 13 46
APOA4 Apolipoprotein A-IV P06727 45.30 5.28 22 50
FETUA Alpha-2-HS-glycoprotein P02765 40.00 5.43 10 38
HPT Haptoglobin P00738 45.80 6.13 20 44
IGHG1 Ig gamma-1 chain C region P01857 36.60 8.46 8 30
IGKC Ig kappa chain C region P01834 11.77 5.58 4 64
PGDS Prostaglandin-H2 D-isomerase P41222 21.01 7.66 12 55
TRFE Serotransferrin P02787 79.29 6.81 24 36
TTHY Transthyretin P02766 15.87 5.52 10 73
  1. a. Protein name and accession number according to the SWISS-PROT databases.
  2. b. Predicted molecular weights in kilodaltons and pI on SWISS-PROT database.
  3. c. Number of peptide masses matching the top hit from MS-Fit PMF.
  4. d. The percentage of sequence coverage of matched peptides in the identified protein.