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Figure 2 | Proteome Science

Figure 2

From: Fetal calf serum heat inactivation and lipopolysaccharide contamination influence the human T lymphoblast proteome and phosphoproteome

Figure 2

Phospho-specific florescence stained 2-DE. (A) Proteins were resolved on 2-DE and gels were stained by Pro-Q Diamond phospho-stain (Invitrogen) and then scanned (FLA -5100). The spots showing significant regulation after densitometry analysis were marked and identified by Q-TOF MS/MS analysis. (B) Illustration of two representative 2-DE spots (TCTP, spot 16; ACTB; spot 17) in non-heated FCS with normal LPS (NHE) and in heated FCS with normal LPS (HE). The error bars represent mean ± SD (*= p < 0.05) of six independent experiments.

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Proteome Science

ISSN: 1477-5956