Fetal calf serum heat inactivation and lipopolysaccharide contamination influence the human T lymphoblast proteome and phosphoproteome

Table 1 Differentially regulated proteins by LPS and heat inactivation of FCS

Spot ID	Abb.	Protein name^a	Acc. No.	Mass (kDa)	Fold change (Mean ± SD)
					HE/NHE	NHL/NHE	HL/NHE	NHL/HE	HL/HE
1	SYFB	PhenylalanyltRNA synthetase beta chain	Q9NSD9	66.1	NS	*2.35 ↓ (0.019 ± 0.01/0.04 ± 0.02)	NS	NS	**2.07 ↓ (0.018 ± 0.007/ 0.037 ± 0.008)
2	QCR1	Cytochrome b-c1 complex subunit 1	P31930	52.6	NS	NS	NS	NS	*1.73 ↓ (0.042 ± 0.012/ 0.074 ± 0.018)
3	SUCB1	Succinyl-CoA ligase subunit beta	Q9P2R7	50.3	NS	NS	NS	NS	*1.50 ↓ (0.55 ± 0.007/ 0.083 ± 0.017)
4	EIF3M	Eukaryotic translation initiation factor 3 subunit M	Q7L2H7	42.5	**1.79 ↑ (0.103 ± 0.013/ 0.057 ± 0.011)	NS	NS	NS	NS
5	NAGK	N-acetyl-D-glucosamine kinase	Q9UJ70	37.3	NS	NS	*1.60 ↑ (0.064 ± 0.024/ 0.04 ± 0.007)	NS	NS
6	PRS7	26S protease regulatory subunit 7	P35998	48.6	*1.69 ↑ (0.104 ± 0.018/ 0.061 ± 0.035)	NS	NS	NS	NS
7	PSB4	Proteasome subunit beta type-4	P28070	29.2	*1.53 ↑ (0.087 ± 0.022/ 0.057 ± 0.017)	NS	NS	NS	NS
8	MOBKL1A	Mps one binder kinase activator A1	Q7L9L4	25.0	NS	NS	NS	**1.85 ↑ (0.076 ± 0.0150/ 0.04 ± 0.009)	NS
9	SOD2	Superoxide dismutase [Mn], mitochondrial	P04179	24.7	NS	NS	NS	*1.68 ↓ (0.08 ± 0.037/ 0.135 ± 0.028)	NS
10	SNAPA	Alpha-soluble NSF attachment protein	P54920	33.2	*1.70 ↑ (0.068 ± 0.015/ 0.04 ± 0.015)	NS	NS	NS	NS
11	DBLOH	Diablo homolog, mitochondrial	Q9NR28	7.1	NS	NS	*2.02 ↑ (0.074 ± 0.008/ 0.033 ± 0.019)	NS	NS

Abb: Abbreviation; Acc. No: Accession number; Mass: Molecular mass of the protein observed in a Mascot search (www.matrixscience.com);
^a Proteins identified by Q-TOF MS/MS analysis and database search against Swissprot; ↑: Up-regulated, ↓: Down-regulated, NS: Non-significant change; *= p < 0.05, **= p < 0.005.

ISSN: 1477-5956