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Table 2 Differentially phosphorylated proteins by LPS and heat inactivation of FCS

From: Fetal calf serum heat inactivation and lipopolysaccharide contamination influence the human T lymphoblast proteome and phosphoproteome

Spot ID

Abb.

Protein name a

Acc. No.

Mass

(kDa)

Fold change (Mean ± SD)

     

HE/NHE

NHL/NHE

HL/NHE

NHL/HE

HL/HE

12

TCPD

T-complex protein 1 subunit delta

P50991

57.8

*1.62 ↑ (0.219 ± 0.047/0.135 ± 0.053)

NS

NS

*1.68 ↓ (0.130 ± 0.068/0.219 ± 0.046)

NS

13

ACTA

Actin, aortic smooth muscle

P62736

41.9

*1.83 ↑ (0.108 ± 0.036/0.059 ± 0.029)

NS

NS

NS

NS

14

ADHX

Alcohol dehydrogenase class-3

P11766

39.6

NS

NS

NS

NS

*1.75 ↑ (0.226 ± 0.08/0.129 ± 0.046)

15

NACA

Nascent polypeptide-associated complex subunit alpha

Q13765

23.3

*2.60 ↑ (0.283 ± 0.095/0.108 ± 0.031)

NS

NS

*1.74 ↓ (0.162 ± 0.044/0.283 ± 0.095)

NS

16

TCTP

Translationally-controlled tumor protein

P13693

19.5

*2.30 ↑ (0.073 ± 0.013/0.031 ± 0.015)

NS

NS

NS

NS

17

ACTB

Actin, cytoplasmic 1

Q96HG5

41.7

*2.2 0 ↑ (0.062 ± 0.015/0.028 ± 0.009)

NS

NS

*2.37 ↓ (0.018 ± 0.011/0.043 ± 0.015)

NS

18

ICLN

Methylosome subunit pICln

P54105

26.1

*2.08 ↑ (0.232 ± 0.089/0.111 ± 0.081)

NS

NS

*1.80 ↓ (0.283 ± 0.117/0.510 ± 0.113)

NS

  1. Abb: Abbreviation; Acc. No: Accession number; Mass: Molecular mass of the protein observed in MASCOT search; a Phospho-proteins identified by Q-TOF MS/MS analysis and database search against Swissprot; ↑: Up-regulated, ↓: Down-regulated, NS: Non-significant change; *= p < 0.05, **= p < 0.005.