Protein profiles. A) Profiles before any data processing are shown from four antibodies across all samples. B) Profiles for the antibodies are shown after normalization, removal of outliers and BoxCox transformation. The red line indicates the locally weighted scatterplot smoothing (LOWESS). The vertical dashed lines differentiate between three microtiter plates in which the samples were distributed. Graphs from all antibodies pre and post data treatment are found in Additional Files 1 and 2.