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Table 1 List of identified proteins.

From: S100A10 protein expression is associated with oxaliplatin sensitivity in human colorectal cancer cells

Mass spectrum data: protein data of all 2-DE spots selected, peptide data from spot 4 and sequence coverage of spot 4 (Protein S100-A10).

Protein Data

  

Physical data

MS/MS ion search results

Characterization of 11.1 kDa protein by SELDI-TOF MS

  

Swiss-Prot

In gel digestion from 2-DE

  

Spot (s)

Protein name

Swiss-Prot Accession No.

Theoretical molecular weight using Expasy tool (Da)

Theoretical pI using Expasy tool

Sequence coverage

(%)

Mowse score

Observed molecular mass

( m/z )

Observed pI

1

Protein S100-A11

P31949

11741

6.55

26

78

  

2

No hit

-

-

-

-

-

  

3

Beta-2 microglobulin

P61769

11731

6.08

17

65

  

4

Protein S100-A10

P60903

11072

7.31

10

38

11072

7.0-7.5

Peptide data from spot 4 (Protein S100-A10)

 

Start - End

Observed

Mr (expt)

Mr (calc)

Delta

Miss

Sequence

 

18 - 27

550.29

1098.57

1098.57

0.00

1

K.FAGDKGYLTK.E

Sequence coverage

 

Matched peptide is underlined

1 PSQMEHAMET MMFTFHKFAG DKGYLTK EDL RVLMEKEFPG FLENQKDPL

51 VDKIMKDLDQ CRDGKVGFQS FFSLIAGLTI ACNDYFVVHM KQKGKK

  1. Mowse score > 30 indicates identity or extensive homology (p < 0.05). Search parameters: MS/MS ion search, enzyme: trypsin, fixed modificaton: carbamidomethyl (C), variable modification: oxidation (M), peptide tolerance: ± 0.05 Da, fragment mass tolerance: ± 0.05 Da, max missed cleavage: 1.