Mass spectrum data: protein data of all 2-DE spots selected, peptide data from spot 4 and sequence coverage of spot 4 (Protein S100-A10). | ||||||||
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Protein Data | ||||||||
 |  | Physical data | MS/MS ion search results | Characterization of 11.1 kDa protein by SELDI-TOF MS | ||||
 |  | Swiss-Prot | In gel digestion from 2-DE |  |  | |||
Spot (s) | Protein name | Swiss-Prot Accession No. | Theoretical molecular weight using Expasy tool (Da) | Theoretical pI using Expasy tool | Sequence coverage (%) | Mowse score | Observed molecular mass ( m/z ) | Observed pI |
1 | Protein S100-A11 | P31949 | 11741 | 6.55 | 26 | 78 | Â | Â |
2 | No hit | - | - | - | - | - | Â | Â |
3 | Beta-2 microglobulin | P61769 | 11731 | 6.08 | 17 | 65 | Â | Â |
4 | Protein S100-A10 | P60903 | 11072 | 7.31 | 10 | 38 | 11072 | 7.0-7.5 |
Peptide data from spot 4 (Protein S100-A10) | ||||||||
 | Start - End | Observed | Mr (expt) | Mr (calc) | Delta | Miss | Sequence | |
 | 18 - 27 | 550.29 | 1098.57 | 1098.57 | 0.00 | 1 | K.FAGDKGYLTK.E | |
Sequence coverage | ||||||||
 | Matched peptide is underlined 1 PSQMEHAMET MMFTFHKFAG DKGYLTK EDL RVLMEKEFPG FLENQKDPL 51 VDKIMKDLDQ CRDGKVGFQS FFSLIAGLTI ACNDYFVVHM KQKGKK |