Analysis of protein composition of rabbit aqueous humor following two different cataract surgery incision procedures using 2-DE and LC-MS/MS

Stastna, Miroslava; Behrens, Ashley; McDonnell, Peter J; Van Eyk, Jennifer E

doi:10.1186/1477-5956-9-8

Proteome Science

Table 2 Protein spot changes from 0.5 hour to 2.0 hour time points within clear corneal (A) and limbal (B) incision procedures.

From: Analysis of protein composition of rabbit aqueous humor following two different cataract surgery incision procedures using 2-DE and LC-MS/MS

	spot # (observed pI/M_w [kDa])	fold change	p-value	proteins identified (accession #; species; theoretical pI/M_w [Da]; PIP [%])
				at 0.5 hour	at 2.0 hours
A	42078 (5.3/44)	-2.2	0.0068	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 95%)	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 94%)
				Serum albumin (gi\|126723746; O. cuniculus; 5.85/68910; 94%)
				Albumin (gi\|109074537; M. mulatta; 5.91/68874; 95%)
	42147 (6.0/36)	+6.9	0.0040	*	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 93%)
					Iodotyrosine dehalogenase 1 protein (gi\|21312562; M. musculus; 5.97/32814; 89%)
					Mitogen-activated protein kinase 4 (gi\|157819181; R. norvegicus; 7.43/35056; 92%)
	42151 (4.8/50)	+4.5	0.0004	Try10-like trypsinogen (gi\|51092303; M. musculus; 4.83/26531; 95%)	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 94%)
				Collagen, Alpha 3 type VI (gi\|73990557; C. familiaris; 5.43/179948; 88%)	Valosin-containing protein (gi\|11095436; H. sapiens; 6.08/34392; 83%)
					Alpha-2-plasmin inhibitor (gi\|130488651; O. cuniculus; 5.89/54719; 89%)
	42629 (5.1/54)	+2.8	0.0328	Alpha-1-antiproteinase F (gi\|126722912; O. cuniculus; 5.83/45868; 100%)	Alpha-1-antiproteinase F (gi\|126722912; O. cuniculus; 5.83/45868; 100%)
					SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 95%)
					SK2 (gi\|73970454; C. familiaris; 9.78/26354; 83%)
					Olfactory receptor, family 2, member 35 (gi\|49226830; H. sapiens; 9.03/36101; 85%)
	42696 (5.7/44)	+956	0.0318	**	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 94%)
					Serum albumin (gi\|126723746; O. cuniculus; 5.85/68910; 94%)
					Tudor domain-containing protein 12 (gi\|162416223; M. musculus; 6.11/137627; 84%)
					Paraoxonase (gi\|126722853; O. cuniculus; 5.51/40010; 94%)
B	42064 (5.0/14)	+2.8	0.0039	*	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 95%)
					Bloom syndrome protein (gi\|109082375; M. mulatta; 8.01/158903; 88%)
	42092 (4.1/55)	+3957	0.0450	**	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 90%)
					Iodotyrosine dehalogenase 1 protein (gi\|21312562; M. musculus; 5.97/32814; 84%)
					Alpha -2-HS-glycoprotein (gi\|12644357; O. cuniculus; 4.99/36696; 95%)
					Plasminogen (gi\|38051823; H. sapiens; 6.89/90585; 91%)
					Cytosolic sialic acid 9-O-acetylesterase (gi\|73954581; C. familiaris; 6.27/57141; 83%)
	42605 (6.5/65)	+7.0	0.0371	Serum albumin (gi\|126723746; O. cuniculus; 5.85/68910; 100%)	Serum albumin^a (gi\|126723746; O. cuniculus; 5.85/68910; 100%)
					SLAM family member 9^a (gi\|74760694; H. sapiens; 817/30379; 89%)
					Serum Transferrin Chain A^a (gi\|15825992; O. cuniculus; 6.35/74790; 100%)
					Tudor domain-containing protein 12^a (gi\|162416223; M. musculus; 6.11/137627; 89%)
					Shank-interacting protein-like 1^a (gi\|73974864; C. familiaris; 10.39/73215; 89%)
	42933 (6.3/29)	+3.5	0.0356	*	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 95%)
					Olfactory receptor Olr1474 (gi\|47575923; R. norvegicus; 8.01/35443; 95%)

For each protein the accession number, species, theoretical pI and M_w [Da; average] and protein identification probability [%] are given in parenthesis (two last columns of the table). The theoretical pI and M_w were computed by using Expasy Proteomics server (Swiss Institute of Bioinformatics; http://ca.expasy.org/tools/pi_tool.html).
For each spot the pI and M_w [kDa] as observed in 2-DE gels are given in parenthesis (2^nd column of the table).
*very faint protein spot in 2-DE gel - no proteins identified
**no spot detected by gel image analysis
^aproteins identified at 12 hour time point

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ISSN: 1477-5956

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	spot # (observed pI/M_w [kDa])	fold change	p-value	proteins identified (accession #; species; theoretical pI/M_w [Da]; PIP [%])
				at 0.5 hour	at 2.0 hours
A	42078 (5.3/44)	-2.2	0.0068	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 95%)	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 94%)
				Serum albumin (gi\|126723746; O. cuniculus; 5.85/68910; 94%)
				Albumin (gi\|109074537; M. mulatta; 5.91/68874; 95%)
	42147 (6.0/36)	+6.9	0.0040	*	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 93%)
					Iodotyrosine dehalogenase 1 protein (gi\|21312562; M. musculus; 5.97/32814; 89%)
					Mitogen-activated protein kinase 4 (gi\|157819181; R. norvegicus; 7.43/35056; 92%)
	42151 (4.8/50)	+4.5	0.0004	Try10-like trypsinogen (gi\|51092303; M. musculus; 4.83/26531; 95%)	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 94%)
				Collagen, Alpha 3 type VI (gi\|73990557; C. familiaris; 5.43/179948; 88%)	Valosin-containing protein (gi\|11095436; H. sapiens; 6.08/34392; 83%)
					Alpha-2-plasmin inhibitor (gi\|130488651; O. cuniculus; 5.89/54719; 89%)
	42629 (5.1/54)	+2.8	0.0328	Alpha-1-antiproteinase F (gi\|126722912; O. cuniculus; 5.83/45868; 100%)	Alpha-1-antiproteinase F (gi\|126722912; O. cuniculus; 5.83/45868; 100%)
					SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 95%)
					SK2 (gi\|73970454; C. familiaris; 9.78/26354; 83%)
					Olfactory receptor, family 2, member 35 (gi\|49226830; H. sapiens; 9.03/36101; 85%)
	42696 (5.7/44)	+956	0.0318	**	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 94%)
					Serum albumin (gi\|126723746; O. cuniculus; 5.85/68910; 94%)
					Tudor domain-containing protein 12 (gi\|162416223; M. musculus; 6.11/137627; 84%)
					Paraoxonase (gi\|126722853; O. cuniculus; 5.51/40010; 94%)
B	42064 (5.0/14)	+2.8	0.0039	*	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 95%)
					Bloom syndrome protein (gi\|109082375; M. mulatta; 8.01/158903; 88%)
	42092 (4.1/55)	+3957	0.0450	**	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 90%)
					Iodotyrosine dehalogenase 1 protein (gi\|21312562; M. musculus; 5.97/32814; 84%)
					Alpha -2-HS-glycoprotein (gi\|12644357; O. cuniculus; 4.99/36696; 95%)
					Plasminogen (gi\|38051823; H. sapiens; 6.89/90585; 91%)
					Cytosolic sialic acid 9-O-acetylesterase (gi\|73954581; C. familiaris; 6.27/57141; 83%)
	42605 (6.5/65)	+7.0	0.0371	Serum albumin (gi\|126723746; O. cuniculus; 5.85/68910; 100%)	Serum albumin^a (gi\|126723746; O. cuniculus; 5.85/68910; 100%)
					SLAM family member 9^a (gi\|74760694; H. sapiens; 817/30379; 89%)
					Serum Transferrin Chain A^a (gi\|15825992; O. cuniculus; 6.35/74790; 100%)
					Tudor domain-containing protein 12^a (gi\|162416223; M. musculus; 6.11/137627; 89%)
					Shank-interacting protein-like 1^a (gi\|73974864; C. familiaris; 10.39/73215; 89%)
	42933 (6.3/29)	+3.5	0.0356	*	SLAM family member 9 (gi\|74760694; H. sapiens; 8.17/30379; 95%)
					Olfactory receptor Olr1474 (gi\|47575923; R. norvegicus; 8.01/35443; 95%)