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Table 3 Protein spot changes between clear corneal and limbal incisions at 0.5 hour (A) and 2 hour (B) time points.

From: Analysis of protein composition of rabbit aqueous humor following two different cataract surgery incision procedures using 2-DE and LC-MS/MS

 

spot # (observed pI/Mw [kDa])

fold change

p-value

proteins identified

(accession #; species; theoretical pI/Mw [Da]; PIP [%])

    

clear corneal incision

limbal incision

A

42558

(6.2/60)

-1.6

0.0219

SLAM family member 9

(gi|74760694; H. sapiens; 8.17/30379; 95%)

*

    

Serum albumin

(gi|126723746; O. cuniculus; 5.85/68910; 100%)

 
    

Mucin 16

(gi|34501467; H. sapiens; 5.51/744966; 85%)

 

B

42543

(5.6/18)

+2.8

0.0366

SLAM family member 9

(gi|74760694; H. sapiens; 8.17/30379; 95%)

SLAM family member 9

(gi|74760694; H. sapiens; 8.17/30379; 93%)

    

FERM

(gi|73994333; C. familiaris; 9.13/117880; 95%)

Serum albumin

(gi|126723746; O. cuniculus; 5.85/68910; 100%)

    

GTP-binding protein Mx2

(gi|7271909; C. familiaris; 8.33/81442; 90%)

E2F transcription factor 4

(gi|109128874; M. mulatta; 4.66/44295; 92%)

 

42727

(5.5/18)

-2.2

0.0012

SLAM family member 9

(gi|74760694; H. sapiens; 8.17/30379; 95%)

*

    

Iodotyrosine dehalogenase 1 protein

(gi|21312562; M. musculus; 5.97/32814; 88%)

 
 

43222

(4.2/65)

+2697

0.0095

**

SLAM family member 9

(gi|74760694; H. sapiens; 817/30379; 89%)

     

Serum albumin

(gi|126723746; O. cuniculus; 5.85/68910; 100%)

     

HIST2H3C protein

(gi|55626038; P. troglodytes; 10.50/60795; 100%)

     

Desmoglein 4

(gi|148664532; M. musculus; 4.61/102815; 100%)

  1. For each protein the accession number, species, theoretical pI and Mw [Da; average] and protein identification probability [%] are given in parenthesis (two last columns of the table). The theoretical pI and Mw were computed by using Expasy Proteomics server (Swiss Institute of Bioinformatics; http://ca.expasy.org/tools/pi_tool.html).
  2. For each spot the pI and Mw [kDa] as observed in 2-DE gels is given in parenthesis (2nd column of the table).
  3. *very faint spot in 2-DE gel - no proteins identified
  4. **no spot detected by gel image analysis