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Table 2 The m/z and RT values of the spike-in peptides in our in-house LC-MALDI-TOF dataset before and after alignment. The ranges of RT before alignment were obtained from the MALDI-TOF-TOF spectra of the samples with spike-in peptides.

From: A new method for alignment of LC-MALDI-TOF data

 

Original (before alignment)

After alignment

Spiked-in peptides

m/z

(Dalton)

RT

(fraction)

m/z

(Dalton)

RT

(fraction)

Angiotensin

1296.7

41-44

1296.03

52

Glu1-Fibrinopeptide B

1570.7

31-34

1570.65

35

ACTH 1-17 fragment

2093.1

22-24

2093.97

30

ACTH 18–39 fragment

2465.2

55-60

2465.13

63