Two schemes for computation saving. Two schemes for reducing the computation of the subcellular localization process. In Scheme I, a full-length query sequence is presented to PSI-BLAST for computing a full-length profile; then the profile is truncated at the predicted cleavage site. The truncated profile is then aligned with all of the truncated training profiles to produce a profile-alignment score vector for classification. In Scheme II, the query sequence is truncated at the predicted cleavage site before inputting to PSI-BLAST for computing the profile. The cleavage sites are predicted by CSitePred  or TargetP .