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Figure 1 | Proteome Science

Figure 1

From: New insights in the composition of extracellular vesicles from pancreatic cancer cells: implications for biomarkers and functions

Figure 1

EVs from pancreatic cancer cells: a) Immunoblots of different samples: proteins characteristic for EVs were enriched in pellets (P) after ultrafiltration and ultracentrifugation, compared to secretome (Secr) or lysate samples (Ly). b) The crude extracellular vesicle samples in an OptiPrep gradient centrifugation. The EV markers Syntenin and CD63/Lamp3 were found in fractions with densities of 1.07-1.15 g/ml as illustrated by dot blots. c) Transmission electron microscope (TEM) pictures of 120,000 g pellets of Panc1 and Paca44. Lipid bilayers surrounding the vesicles are visible (arrowheads).

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Proteome Science

ISSN: 1477-5956