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Figure 8 | Proteome Science

Figure 8

From: New insights in the composition of extracellular vesicles from pancreatic cancer cells: implications for biomarkers and functions

Figure 8

Release of different amounts of CD73/NT5E proteins: a) NT5E/CD73 was detected in EV samples of pancreatic carcinoma cell lines in different amounts. No NT5E/CD73 was found in the samples of the normal model cells HPDE. The corresponding silver stained gel part is shown for the control. b) Ascites derived from PDAC and CRC (colorectal carcinoma) patients and the conditioned medium (CM) of primary cancer cells (isolated from ascites) were used to prepare the EV samples. Samples were tested for NT5E/CD73 by western blot. Additionally, samples of the CRC patients were checked. c) Human pancreatic stellate cells (HPSC) also release some of the EV proteins, but in a lesser amount than the pancreatic cancer cell line. An example of corresponding silver stained gel is given. Ly, lysate; Secr, secretome; EV, EV crude preparation.

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