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Table 2 Assay linearity

From: Mass Spectrometric Immunoassay for the qualitative and quantitative analysis of the cytokine Macrophage Migration Inhibitory Factor (MIF)

Sample

Dilution

Observed

Expected

Recovery

  

ng/mL

ng/mL

O/E %

1

 

31.5

  
 

2×

15.5

15.7

98.3

 

4×

7.36

7.87

93.5

 

8×

4.20

3.93

107

2

 

29.2

  
 

2×

13.6

14.6

93.2

 

4×

7.00

7.30

95.9

 

8×

4.20

3.65

115

  1. Serum samples were, on two separate days, serially diluted 2×, 4× and 8× and MSIA was performed. Intact MIF concentrations were determined from accompanying standard curve.
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