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Figure 2 | Proteome Science

Figure 2

From: Promoter trapping method: transcription factor purification using human telomerase reverse transcriptase promoter

Figure 2

Analysis of hTERT promoter activity using dual luciferase assay. hTERT was subcloned from hTERT-pUC19 into pMLUC. During transfection, pTK-LUC and hTERT-pMLUC or the empty vector control were mixed and transfected into cell line HEK293. Cells were lysed 48 hours later and acitivity was measured with a dual luciferase assay. Two measurements were taken, renilla and firefly luciferase, and the ratio of the two was used to measure the activity of each condition. The average of triplicates (from top to bottom) were 1.9, 62733, 13.7, 50, and 160.6. cJun is 4579 times higher activity than hTERT.

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Proteome Science

ISSN: 1477-5956