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Figure 5 | Proteome Science

Figure 5

From: Promoter trapping method: transcription factor purification using human telomerase reverse transcriptase promoter

Figure 5

MS/MS fragmentation of peptides corresponding to AP2C_HUMAN and AP2D_HUMAN. Transcription factor activator protein 2 (AP-2) was identified by LC/MSMS and was matched to two different isoforms. AP-2-gamma (AP2C) was identified from m/z = 454.25 (3+) and AP-2-delta (AP2D) from m/z = 380.23 (2+) by protein database searching with Mascot software utilizing the SwissProt database. AP2C was identified with a Mascot protein score of 25 and sequence coverage of 3% and 45% probability while AP2D was identified with a Mascot protein score of 33.5 and 4% sequence coverage and 89% probability. The top-ranked tryptic peptide from AP2D contained amino acid VTIAEVK, spanning amino acid residues 229-235 with an expectation value of 0.002. The top-ranked tryptic peptide from AP2C contained amino acid KNMLLAAQQLCK, spanning amino acid residues 349-360 with an expectation value of 0.0007.

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