Figure 6

Transcription characterization with 1-D WB. TF characterization with one-dimensional Western blotting (1-D WB). 1D-SDS-PAGE gel was electro-blotted onto a PVDF membrane and then probed with the following antibodies: TATA binding protein (TBP), RNA Polymerase II (Pol II), upstream stimulatory factor 2 (USF2), specificity protein 1 (SP1), and ßactin. The individual lanes of the SDS-PAGE gel were loaded with whole cell lysate (WCL, protein from the cytosol as well as nucleus) obtained from sonication followed by centrifugation to remove cell debris; nuclear extract (NE); hTERT promoter trapping eluent (PTE). Bands indicate components that are specific to the hTERT promoter as well as demonstrate purification of specific TF from the complex mixtures of WCL and NE.