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Figure 2 | Proteome Science

Figure 2

From: Effects of mycophenolate mofetil on kidney function and phosphorylation status of renal proteins in Alport COL4A3-deficient mice

Figure 2

Differentially phosphorylated protein spots in fibrotic kidneys reversed by mycophenolate mofetil. A) Fluorescence: The point diagram presents the percentage of the relative fluorescence of the whole gel (100%), as well as stratification for all three experimental groups (WT n = 3 in gray; PLC n = 3 in black; MMF n = 3 in white). B) Cluster check: The hit phosphospots are shown as squares in different colors, depending on the fluorescence in the original scans of the phospho-stained 2DE gels (using the analysis tool of the Delta2D software). The stratification is done for all three experimental groups (WT n = 3; PLC n = 3; MMF n = 3). C) Silver-stained 2DE gel: The picture visualizes the phosphospots of one representative WT mouse, as well as the isoelectric point (pI) and the molecular weight (kDa) in this zoomed-in area. Legends: wild-type 129/SvJ mice (WT); placebo treated COL4A3−/− mice (PLC); and COL4A3−/− mice treated with 100 mg/kg mycophenolate mofetil per day (MMF).

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