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Table 1 Affected proteins tentatively identified in sensitive leaves infiltrated with Ptr ToxA

From: Proteome changes induced by Pyrenophora tritici-repentis ToxA in both insensitive and sensitive wheat indicate senescence-like signaling

Spot (1)

Putative Identity

Identification

Taxonomy

Sequence (2)

Score (3)

Ratio toxin:control (4)

Trend

Day 1

Day 2

Day 3

5

Ferredoxin-NADP(H) oxidoreductase

gi 20322473

Triticum aestivum

LYSIASSALGDFGDSK

114

0.485

0.368

0.295

Decrease

DPNATIIMLATGTGIAPFR

111

TOTAL

1278

8

putative GTP-binding protein typA

gi 50906979

Oryza sativa

INIIDTPGHSDFGGEVER

89

0.348

0.336

0.183

Decrease

ALEFGHAVVVVVNK

107

TOTAL

784

28

ATP-dependant Clp protease ATP-binding subunit precursor

gi 26518520

Oryza sativa

LDMSEYMER

75

4.470

35.726

8.728

Increase

RKPFTVVLLDEIEK

81

TOTAL

732

29

ATP-dependant Clp protease ATP-binding subunit precursor

gi 26518520

Oryza sativa

GELQCIAATTLDEHR

94

3.316

5.155

4.355

Increase

GALDQFCLDLTTQASGGFIDPIIGREEEIER

100

TOTAL

731

21

Methionine synthase 2 enzyme

gi 68655500

Hordeum vulgare

GMLTGPVTILNWSFVR

116

2.455

5.030

35.526

Increase

ALAGQKDEAYFAANAAAAQASR

116

TOTAL

2165

34

putative Ado Met synthase 3

gi 68655446

Hordeum vulgare

NIGFISDDVGLDADR

122

0.533

0.800

1.715

Increase

ENFDFRPGMISINLDLKK

123

TOTAL

1475

53

Cinnamyl alcohol dehydrogenase 2a

gi 15428280

Festuca arundinacea

LVLMGVIAEPLSFVSPMVMLGR

69

0.509

2.196

3.536

Increase

IPAGLAPEQAAPLLCAGVTVYSPLK

85

TOTAL

633

66

Glutamine synthase isoform GS1b

gi 71361902

Triticum aestivum

IIAEYIWIGGSGMDLR

100

0.964

0.929

3.011

Increase

HETADINTFSWGVANR

100

TOTAL

1256

  1. (1)Spot numbers correspond to numbers in Figure 3.
  2. (2)The two most significant peptide sequences are shown.
  3. (3)Mascot Ions scores are −10.Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 47 indicate identity or extensive homology (p < 0.05) [46].
  4. (4)Quantitative values are explained in the text and in Additional file 1: Table S1.