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Figure 4 | Proteome Science

Figure 4

From: Interaction of peroxiredoxin V with dihydrolipoamide branched chain transacylase E2 (DBT) in mouse kidney under hypoxia

Figure 4

Comparative interactions of Prdx V WT or cysteine mutants with DBT in normoxic and hypoxic cells. (A) All WCE presented for every Prdx V mutant showed the same background of cellular expression for each transfected construct. (B) Coprecipitation of HA-tagged Prdx V with myc-tagged DBT. (C) Reverse immunoprecipitation of myc-tagged DBT with HA-tagged Prdx V. HEK293 cells were cotransfected with the HA-tagged Prdx V WT or cysteine mutant (C48S, C73S, C152S, C48/152S) and myc-tagged DBT expression vector, and then incubated under normoxic and hypoxic conditions. WCE, whole cell lysate of HEK293 cell; Mock, the empty vector tagged to myc or HA.

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Proteome Science

ISSN: 1477-5956