Figure 2

Interaction networks of the retrieved Nek4.1 and Nek4.2 interactors from IP-MS. The selected most relevant enriched GO biological processes are depicted in (A) Nek4.1 and (B) Nek4.2 networks by clustering the proteins involved in each of the biological processes with a circle layout. Clusters were assigned only to enriched biological processes containing at least two proteins (biological processes of specific cell types were not considered); proteins belonging to more than one biological process were assigned to clusters with the best enrichment p-values. More specific biological processes are shown only for proteins with more specific annotation in GO database. The red nodes correspond to both Nek4 isoforms, the cyan nodes correspond to proteins identified in more than 2 IP-MS biological replicates (from 2 to 4 experiments) and yellow nodes correspond to proteins identified in only one biological replicate. In (A) Nek4.1 network, TRAP1 was assigned to “Apoptotic process” according to Montesano and co-workers [30]; and 5 proteins involved in different cilium functions are depicted inside a blue circle: DFNB31, IFT172, MYO7A, RPGRIP1L and TTC21B [17,31-34]. The protein-protein interaction networks were built using the IIS platform and visualized using the Cytoscape software.