Figure 3

Quantitative immunoblot analysis of selected proteins in SKF38393- and vehicle-treated gerbils. Gerbils received bilateral local injections of SKF38393 or vehicle into the auditory cortex and were decapitated 24 h later. A. Western blots of the synaptic junctional protein-enriched fraction from auditory cortex probed with antibodies against hnRNP K, β-actin, and hnRNP L. B. Western blot of the triton-soluble protein fraction from auditory cortex probed with antibodies against α-synuclein and β-actin. C. Western blot of the triton-soluble protein fraction from hippocampus probed with antibodies against α-synuclein and β-actin. Left: immunoblots with ECL signals. Right: optical densities of ECL signals from SKF38393-treated gerbils (filled bars) relative to those from vehicle-treated controls (empty bars). Means + S.E.M.; n = 3–6 per group; *P < 0.05 significantly different from controls (Mann–Whitney U test).