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Figure 4 | Proteome Science

Figure 4

From: Differential effects of dopamine signalling on long-term memory formation and consolidation in rodent brain

Figure 4

Immunocytochemical analysis of bassoon and α-synuclein co-localisation in control- and SKF38393-treated primary hippocampal neurons in culture. In four independent experiments, cultures of DIV21 rat hippocampal neurons were incubated for 3 h in replaced medium containing either no SKF38393 (A-C, control-treatment) or 100 μM SKF38393 (D-F), fixed, stained with bassoon and α-synuclein antibodies, and visualised using confocal laser scanning microscopy. A-F. Representative photomicrographs of neurons stained with bassoon (A, D) and α-synuclein (B, E) immunofluorescence and the merged pictures (C, F) (scale bar: 20 μm). G-H. Merged pictures of bassoon (green) and α-synuclein (red) immunofluorescence signals obtained from dendrites of control- (G) and SKF38393-treated neurons (H) after straightening and deconvolution for co-localisation analysis (scale bar: 10 μm). Arrows in C, F-H paradigmatically indicate co-localisation of bassoon and α-synuclein. I. Co-localisation of bassoon and α-synuclein immunofluorescence signals was quantified in dendrites of ten neurons for each treatment condition from four independent experiments (empty bars: control-treatment; filled bars: SKF38393-treatment). The percentage of bassoon signal co-localised with α-synuclein signal is shown as means + S.E.M. Numbers inside bars are numbers of neuronal dendrites examined. Statistical evaluation was performed using a 2 x 4 (pharmacological treatment x experiment) analysis of variance (ANOVA) and Student’s two-sided t-test for unpaired comparisons. ANOVA values are described in the text. *P < 0.05, significantly different from controls (unpaired t-test).

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