Table 1 Reproducibility of specific protein label-free quantitative phosphorylation analyses from three independent experiments
Human transgelin2 tryptic digest phosphopeptides identified | Site | Calyculin A treated Sample 1 | Calyculin A treated Sample 2 | Calyculin A treated Sample 3 | Avg(Relative. Std) |
|---|---|---|---|---|---|
GPAYGLpSR(+2) | 11th | 62.48 × E06 | 60.18 × E06 | 71.39 × E06 | 64.68 × E06 (0.092) |
GPAYGLpSREVQQK(+2) | 11th | 27.16 × E06 | 27.77 × E06 | 26.94 × E06 | 27.29 × E06 (0.016) |
IQASpTM(ox)AFK(+2) | 84th | 2.41 × E06 | 2.72 × E06 | 2.80 × E06 | 2.64 × E06 (0.078) |
NFpSDNQLQEGK(+2) | 163th | 168.18 × E06 | 193.05 × E06 | 186.64 × E06 | 182.62 × E06 (0.071) |
NFpSDNQLQEGK(+1) | 163th | 3.46 × E06 | 4.93 × E06 | 4.13 × E06 | 4.17 × E06 (0.176) |
NVIGLQMGpTNR(+2) | 180th | 1.94 × E06 | 1.46 × E06 | 1.96 × E06 | 1.79 × E06 (0.158) |
NVIGLQM(ox)GpTNR(+2) | 180th | 1.97 × E06 | 2.52 × E06 | 2.38 × E06 | 2.29 × E06 (0.125) |
GApSQAGMTGYGMPR(+2) | 185th | 62.43 × E06 | 55.27 × E06 | 61.93 × E06 | 59.88 × E06 (0.067) |
GApSQAGM(ox)TGYGMPR(+2) | 185th | 73.58 × E06 | 80.29 × E06 | 77.44 × E06 | 77.10 × E06 (0.044) |
GApSQAGMTGYGM(ox)PR(+2) | 185th | 43.92 × E06 | 43.28 × E06 | 42.38 × E06 | 43.19 × E06 (0.018) |
GApSQAGM(ox)TGYGM(ox)PR(+2) | 185th | 72.91 × E06 | 82.53 × E06 | 107.47 × E06 | 87.64 × E06 (0.204) |