Immunoblot and quantitative RT-PCR analysis of the 20 representative DEPs. The expression profile for Rubisco was detected using 1-D Western blot analysis (A). The identified three different members of Rubisco large subunit genes were up-regulated at transcriptional level in the Bt-transgenic lines (B). The changed patterns of genes for the five DEPs related to photosynthesis were determined (C). The gene expression patterns of the other 12 representative DEPs in both the Bt-transgenic (right) and non-transgenic (left) plants were highlighted (D). The primers used for qRT-PCR were provided in Additional file 6.