Table 1 Proteins identified by MALDI TOF/TOF MS from transgenic cotton leaves
From: Comparative proteomics of Bt-transgenic and non-transgenic cotton leaves
Spot no a. | GI no. b | Function category protein name | Plant species | Exper. c pI/Mr | Thero. d pI/Mr | M P e | SC% f | M. S. g | Relative change (WT/BT) h |
|---|---|---|---|---|---|---|---|---|---|
Posttranslational modification, protein turnover, chaperones (O) | |||||||||
1 | 255556934 | ATPase | R. communis | 5.49/90 | 5.13/90.1 | 11 | 21 | 324 |
|
7 | 147819511 | Hypothetical protein | V. vinifera | 4.86/72 | 5.20/61.4 | 5 | 10 | 256 |
|
9 | 225433375 | Chaperonin | V. vinifera | 5.40/63 | 5.85/61.7 | 6 | 14 | 204 |
|
16 | 12620883 | Rubisco activase | G. hirsutum | 4.99/49 | 5.06/48.6 | 13 | 41 | 822 |
|
27 | 14594915 | Proteasome subunit | N. tabacum | 5.77/27 | 6.12/18.2 | 3 | 32 | 113 |
|
Carbohydrate transport and metabolism (G) | |||||||||
2 | 110224784 | Transketolase | P. acerifolia | 5.73/76 | 6.25/26.0 | 2 | 11 | 82 |
|
3 | 255541252 | Transketolase | R. communis | 5.80/76 | 6.52/81.6 | 5 | 8 | 277 |
|
4 | 255541252 | Transketolase | R. communis | 5.95/76 | 6.52/81.6 | 5 | 8 | 262 |
|
5 | 255541252 | Transketolase | R. communis | 5.89/76 | 6.52/81.6 | 6 | 10 | 404 |
|
6 | 255541252 | Transketolase | R. communis | 6.05/76 | 6.52/81.6 | 6 | 11 | 337 |
|
8 | 3560664 | Rubisco | C. ensifolium | 5.73/66 | 6.4/49.7 | 5 | 13 | 211 |
|
14 | 11230404 | Rubisco, large subunit | C. Pettersson | 5.96/53 | 5.96/52.9 | 12 | 40 | 998 |
|
15 | 33415263 | Enolase | G. hirsutum | 5.69/49 | 6.16/47.9 | 11 | 36 | 502 |
|
22 | 329317332 | Rubisco, large subunit | G. barbadense | 6.20/35 | 6.00/53.7 | 5 | 16 | 326 |
|
24 | 449442663 | Phosphoglycolate phosphatase | C. sativus | 5.58/32 | 6.47/41.7 | 8 | 26 | 442 |
|
26 | 1881499 | Rubisco, large subunit | P. pendula | 6.24/30 | 6.61/52.6 | 9 | 22 | 366 |
|
32 | 548699 | Rubisco, large chain | (−) | 6.40/19 | 6.12/52.6 | 4 | 9 | 104 |
|
Energy production and conversion (C) | |||||||||
10 | 40850676 | Betaine-aldehyde dehydrogenase | G. hirsutum | 5.43/63 | 5.6/55.4 | 5 | 12 | 124 |
|
11 | 91208909 | ATP synthase, beta subunit | G. hirsutum | 5.33/59 | 5.22/53.6 | 13 | 42 | 939 |
|
20 | 225451308 | Auxin-induced protein | V. vinifera | 6.09/39 | 5.96/37.9 | 1 | 3 | 55 |
|
33 | 315364830 | Ion-sulfur protein | C. lanatus | 6.54/19 | 8.45/24.6 | 3 | 18 | 231 |
|
Cytoskeleton (Z) | |||||||||
17 | 281485191 | actin | P. americana | 5.34/44 | 5.31/41.9 | 12 | 46 | 984 |
|
Amino acid transport and metabolism (E) | |||||||||
18 | 211906462 | Glutamine synthase | G. hirsutum | 5.80/43 | 5.77/39.4 | 5 | 16 | 160 |
|
Coenzyme transport and metabolism (H) | |||||||||
19 | 449433772 | Magnesium-chelatase subunit | C. sativus | 4.98/40 | 5.72/46.0 | 5 | 15 | 150 |
|
21 | 255558669 | Porphobilinogen deaminase | R. communis | 6.07/38 | 6.55/40.3 | 3 | 10 | 50 |
|
Inorganic ion transport and metabolism (P) | |||||||||
23 | 225431122 | Ferredoxin--NADP reductase | V. vinifera | 6.46/34 | 8.91/40.8 | 7 | 22 | 270 |
|
Cell envelope biogenesis, outer membrane (M) | |||||||||
25 | 292668595 | Sanguinarine reductase | E. californica | 5.18/29 | 4.97/29.6 | 2 | 6 | 104 |
|
Nucleotide transport and metabolism (F) | |||||||||
34 | 225457446 | Nucleoside diphosphate kinase, | V. vinifera | 6.41/18 | 9.28/26.0 | 3 | 13 | 147 |
|
No related to COG (NO) | |||||||||
12 | 255558986 | Hypothetical protein | R. communis | 5.39/58 | 8.23/54.9 | 2 | 2 | 69 |
|
13 | 255558986 | Hypothetical protein | R. communis | 5.55/59 | 8.23/54.9 | 2 | 2 | 53 |
|
28 | 226358407 | Chlorophyll binding protein | G. hirsutum | 5.30/27 | 5.53/25.6 | 3 | 20 | 166 |
|
29 | 118489712 | Unknown | P. trichocarpa | 4.73/26 | 4.77/24.4 | 3 | 9 | 156 |
|
30 | 147767601 | Hypothetical protein | V. vinifera | 5.23/42 | 8.46/25.5 | 2 | 11 | 59 |
|
31 | 302595736 | Oxygen-evolving enhancer protein | (−) | 6.26/22 | 8.67/28.2 | 3 | 9 | 215 |
|
35 | 211906510 | Major latex-like protein | G. hirsutum | 5.38/17 | 5.46/17.2 | 3 | 27 | 116 |
|
