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Table 1 Proteins identified by MALDI TOF/TOF MS from transgenic cotton leaves

From: Comparative proteomics of Bt-transgenic and non-transgenic cotton leaves

Spot no a. GI no. b Function category protein name Plant species Exper. c pI/Mr Thero. d pI/Mr M P e SC% f M. S. g Relative change (WT/BT) h
Posttranslational modification, protein turnover, chaperones (O)
1 255556934 ATPase R. communis 5.49/90 5.13/90.1 11 21 324
7 147819511 Hypothetical protein V. vinifera 4.86/72 5.20/61.4 5 10 256
9 225433375 Chaperonin V. vinifera 5.40/63 5.85/61.7 6 14 204
16 12620883 Rubisco activase G. hirsutum 4.99/49 5.06/48.6 13 41 822
27 14594915 Proteasome subunit N. tabacum 5.77/27 6.12/18.2 3 32 113
Carbohydrate transport and metabolism (G)
2 110224784 Transketolase P. acerifolia 5.73/76 6.25/26.0 2 11 82
3 255541252 Transketolase R. communis 5.80/76 6.52/81.6 5 8 277
4 255541252 Transketolase R. communis 5.95/76 6.52/81.6 5 8 262
5 255541252 Transketolase R. communis 5.89/76 6.52/81.6 6 10 404
6 255541252 Transketolase R. communis 6.05/76 6.52/81.6 6 11 337
8 3560664 Rubisco C. ensifolium 5.73/66 6.4/49.7 5 13 211
14 11230404 Rubisco, large subunit C. Pettersson 5.96/53 5.96/52.9 12 40 998
15 33415263 Enolase G. hirsutum 5.69/49 6.16/47.9 11 36 502
22 329317332 Rubisco, large subunit G. barbadense 6.20/35 6.00/53.7 5 16 326
24 449442663 Phosphoglycolate phosphatase C. sativus 5.58/32 6.47/41.7 8 26 442
26 1881499 Rubisco, large subunit P. pendula 6.24/30 6.61/52.6 9 22 366
32 548699 Rubisco, large chain (−) 6.40/19 6.12/52.6 4 9 104
Energy production and conversion (C)
10 40850676 Betaine-aldehyde dehydrogenase G. hirsutum 5.43/63 5.6/55.4 5 12 124
11 91208909 ATP synthase, beta subunit G. hirsutum 5.33/59 5.22/53.6 13 42 939
20 225451308 Auxin-induced protein V. vinifera 6.09/39 5.96/37.9 1 3 55
33 315364830 Ion-sulfur protein C. lanatus 6.54/19 8.45/24.6 3 18 231
Cytoskeleton (Z)
17 281485191 actin P. americana 5.34/44 5.31/41.9 12 46 984
Amino acid transport and metabolism (E)
18 211906462 Glutamine synthase G. hirsutum 5.80/43 5.77/39.4 5 16 160
Coenzyme transport and metabolism (H)
19 449433772 Magnesium-chelatase subunit C. sativus 4.98/40 5.72/46.0 5 15 150
21 255558669 Porphobilinogen deaminase R. communis 6.07/38 6.55/40.3 3 10 50
Inorganic ion transport and metabolism (P)
23 225431122 Ferredoxin--NADP reductase V. vinifera 6.46/34 8.91/40.8 7 22 270
Cell envelope biogenesis, outer membrane (M)
25 292668595 Sanguinarine reductase E. californica 5.18/29 4.97/29.6 2 6 104
Nucleotide transport and metabolism (F)
34 225457446 Nucleoside diphosphate kinase, V. vinifera 6.41/18 9.28/26.0 3 13 147
No related to COG (NO)
12 255558986 Hypothetical protein R. communis 5.39/58 8.23/54.9 2 2 69
13 255558986 Hypothetical protein R. communis 5.55/59 8.23/54.9 2 2 53
28 226358407 Chlorophyll binding protein G. hirsutum 5.30/27 5.53/25.6 3 20 166
29 118489712 Unknown P. trichocarpa 4.73/26 4.77/24.4 3 9 156
30 147767601 Hypothetical protein V. vinifera 5.23/42 8.46/25.5 2 11 59
31 302595736 Oxygen-evolving enhancer protein (−) 6.26/22 8.67/28.2 3 9 215
35 211906510 Major latex-like protein G. hirsutum 5.38/17 5.46/17.2 3 27 116
  1. aAssigned spot number as indicated in Figure 2.
  2. bDatabase GI numbers according to NCBInr.
  3. c,d The experimental (c) and theoretical (d) mass (kDa) and pI of the identified proteins.
  4. eNumber of the matched peptides (MP).
  5. fThe amino acid sequence coverage (SC) for the identified proteins.
  6. gThe Mascot searched score (M. S.) against the database NCBInr.
  7. hThe relatively changed ratios of protein amount on different 2-D gels.