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Figure 1 | Proteome Science

Figure 1

From: Proteomic study of different culture medium serum volume fractions on RANKL-dependent RAW264.7 cells differentiating into osteoclasts

Figure 1

TRAP staining of RANKL-stimulated RAW264.7 cells in medium supplemented with 1% FBS and 10% FBS for 9 consecutive days. Multinucleated osteoclasts were apparently observed on Day 3 in both culture conditions. Osteoclasts formed in medium supplemented with 10% FBS were larger in size than those formed in medium supplemented with 1% FBS. Osteoclast debris was observed from Day 4 in medium supplemented with 10% FBS, but not until Day 6 in the cultures using medium supplemented with 1% FBS.

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