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Table 3 Protein identifications by MS/MS analysis of oocytes which were significantly increased or decreased upon 42O, as compared with 33O

From: Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis

Spot no

Protein name

GI acession

Acession ID

Fold Diff.a

plb

Mwb

Peptides matched

S cov %c

T-test (p)d

Classification

 

Down-regulated

         

1-2

PREDICTED: protein-arginine deiminase type-6

350585686

PADI6

−2.6/-1.73

5.45

79159.2

18

41.723

4.90E-02

enzyme

3

PREDICTED: major vault protein isoform 1

335284397

MVP

−1.85

5.57

99592.5

36

66.638

1.40E-02

Chaperone

4

inositol polyphosphate-1-phosphatase

350529419

INPP1

−1.61

5.21

44472.7

6

19.694

2.10E-02

enzyme

5-6

glucose regulated protein 58

301016769

PDIA3

−2.07/-1.91

5.93

56822.9

21

55.766

4.10E-02

oxidoreductase, isomerase

7

protein,glial fibrillary acidic

223869

GFAP

−1.86

5.96

24946.7

7

3.791

5.70E-03

Cytoskeleton

8

90-kDa heat shock protein

1945447

HSP90B1

−1.52

4.93

84721.7

27

42.224

6.80E-03

Chaperone

9

beta-actin

112980807

ACTG1

−1.55

5.5

29392.7

8

44.348

3.70E-02

Cytoskeleton

 

Up-regulated

         

10

glyceraldehyde-3-phosphate dehydrogenase

229279

GAPDH

1.96

6.9

35686.2

6

14.312

3.00E-02

Metabolism

11-12

DJ-1 protein

67038668

PARK7

1.94/1.52

6.33

19923.5

10

28.298

4.70E-02

Chaperone, oncogene, antioxidation

13

superoxide dismutase [Cu-Zn]

298677090

SOD1

1.74

6.03

15881.9

5

28.238

2.30E-02

Antioxidation

14

glutathione S-transferase mu 2

116047847

GSTM2

1.69

6.9

25746.9

4

4.67

4.80E-02

enzyme

15

PREDICTED: similar to GLUD1 protein

194042282

GLUD1

1.6

10.28

38321.5

13

8.87

2.60E-02

Metabolism

16

eukaryotic translation elongation factor 1 alpha 1

223019599

EEF1A1

2.08

9.1

50109.1

9

8.74

3.40E-02

enzyme/signal transducer

17

PREDICTED: heat shock 90kD protein 1, beta

194039391

HSP90AB1

1.86

4.96

83201.1

27

42.224

2.10E-02

Chaperone

18

PREDICTED: heat shock 70 kDa protein 5

194033595

HSPA5

1.71

5.68

73767.5

24

40.616

1.40E-02

Chaperone

19

PREDICTED: vimentin-like

76097691

VIM

1.71

4.79

34288.2

6

15.727

1.20E-02

Cytoskeleton

20

PREDICTED: ADP-sugar pyrophosphatase-like isoform 1

350589625

NUDT5

1.53

5.14

24253.2

4

31.635

1.00E-02

Metabolism

21

trypsinogen precursor

238866766

PRSS1

1.96

6.85

25864.6

3

10.866

6.60E-03

enzyme

  1. aFold change represents protein upregulate (+) or downregulate (−) in 42O compared with 33O
  2. bPercentage sequence coverage (S cov %) if the mascot score was significant (P < 0.05)
  3. cTheoretical isoelectric point (pI) and theoretical molecular weight (Mw)
  4. d p-value from t-test of the protein level between 33O and 42O