Table 1 Major proteins (≥0.1 %) of the quail eggshell calcified layer

Ovocleidin-116^{a g}

2898a;713b (G1N6E1;OC116)

46.40

31.30

High (65.3)^2a

Ovalbuminⁱ

20482;Q6V115;20852 (G1MYL1;G1MYK6;OVAL)

15.97

12.52

High (85.6)^2a

Ovocalyxin-36^{a g, t}

21806 (G1N6M8;Q53HW8)

7.80

6.00

High (23.2)^2a

Uncharacterized member of the latexin family

11366a

6.32

-

-

Uncharacterized proteinase inhibitor (I1)

23208

3.47

-

-

Avidin/avidin-related proteinⁱ

29257 (G1MSZ9;AVID)

1.95

2.67

Intermediate (2.2)^2a

EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3)^{a i}

25038;28103;20199 (G3UT43; F1NCN3)

1.65

3.70

High (83.3)^2a

Tsukushin^{a g, t}

7859 (G1NRF2;F1NDH7)

1.51

0.25

Intermediate (3.9)^2a

Alpha-2-antiplasmin (SERPINF2)^a

5112;6916 (G1N0Z7;F1NAR5)

0.60

0.80

Low-intermediate^2b,c,d

Gastric intrinsic factor

8574 (G1MW98;R4GLQ4)

0.42

-

-

Serum albumin^{g, t}

16345;19800;4290a (G1NCR2;ALBU;F2Z4L6)

0.39

2.57

High (113.5)^2a

Lactadherin (MFGE8)ⁱ

9870;11245;14016 (G1N944;E1C0K5)

0.36

1.25

High (15.9)^2a

Glutathione peroxidase (GPX3)^{i, t}

19686 (G1N365;F1NPJ8)

0.36

0.04

Intermediate (2.2)^2a

Actin(s)ⁱ

7084 (G1NS52;ACT5;ACTG1))

0.36

-

High (9.0)^2a

Uncharacterized/similar to mucin(−5 AC)

4106;10751;6295;6355 (G1N8Z1;E1C037)

0.35

0.15

Low (1.4)^2a

Uncharacterized/Ovostatin-like (OVSTL)

15509;25086;455a;9460 (G1NK51;F1NEW8)

0.34

0.05

Low (1.5)^2a

Prostate stem cell antigen (PSCA)^{a t}

20430 (G1NJY7;F1NXM7)

0.34

0.18

Low (0.8)^2a

Extracellular fatty acid-binding protein (EXFAB)

Q9I9P7 (G1MQ16;E1C0K1)

0.34

3.82

High (26.8)^2a

Pigment epithelium-derived factor/SERPINF1ⁱ

5860 (G1N131;E1C7H6)

0.31

0.64

Intermediate (6.0)^2a

Clusterin^{a i}

6174;P14018 (G1NMV6;Q9YGP0)

0.31

0.31

High (42.7)^2a

Serpin E2/Glia-derived nexin

6327 (G3URX3;E1BWU2)

0.31

0.18

High (11.1)^2a

Osteopontin^a

Q9I832;713c (G1N6D8;G1N6D8)

0.28

0.15

High (1.89)^2a

Apolipoprotein D (APOD)

28649 (G1N591;Q5G8Y9)

0.27

0.04

High (18.3)^2a

Glypican-4^a

6640 (G1MVZ0;F1NAU1)

0.23

0.12

Intermediate (6.3)^2a

Semaphorin-3G^{g, t}

621a (G1MXI6;F1NQ93)

0.22

0.15

Low (1.7)^2a

Similar to Bactericidal/ permeability-increasing protein-like 2/BPI fold-containing family C protein^{a i, t}

28084;20375 (IPI_CHICK:00571823)

0.21

-

High (16.8)^2a

Pentraxin (PTX3/PPTX)

14241;14979 (G1NDX8;Q5UMH8)

0.21

0.01

Low (0.6)^2a

Ovalbumin-related protein Xⁱ

30155 (G1MZH2;R9TNA6)

0.21

<0.01

Intermediate (3.8)^2a

Ovotransferrin (TRFE)ⁱ

2596;18629;24968 (G1MVV5;E1BQC2;Q4ADJ7)

0.19

2.22

High (22.9)^2a

Cathepsin D

15278;3793b (G1N8P0;CATD)

0.18

0.22

Intermediate (5.3)^2a

Cathepsin B

8924;17826 (G1NN37;F1N9D8)

0.18

0.09

High (24.8)^2a

Sulfhydryl oxidase 1 (QSOX1)ⁱ

2299;5467 (G1MV84;QSOX1;F1NYK2)

0.18

1.08

Intermediate (4.7)^2a

Apolipoprotein A-I^{a g, t}

12041;P32918 (G1MVX1;APOA1)

0.17

0.05

Intermediate (2.2)^2a

Lysozyme C^g

P00701 (LYSC;LYSC)

0.14

0.64

High (128.2)^2a

Fibronectin (FN1)

141;A0A060PIY8 (G1MWJ4;F1NJT3;F1NJT4)

0.13

0.13

High (18.9)^2a

IGFBP5

Q9DGI2 (−;F1ND88)

0.13

-

Low (0.6)^2a

FAM20C kinase/DMP4^a

21781;14673 (G1MSD1;E1C4X0)

0.12

0.27

Intermediate (2.3)^2a

Proactivator polypeptide (PSAP)

2306;1756 (G1MZV1;E1BSP1)

0.12

0.06

High (9.0)^2a

Polyubiquitin/ubiquitin^t

11531;2058 (G1NR52;Q91021;UBB)

0.12

0.12

High (9.0)^2a

Regenerating islet-derived protein 4 (REG4)

8715a (G1MZE6;E1BZV4)

0.11

0.38

Low (0.7)^2a

Cystatin^t

14227 (G1N522;CYT)

0.11

1.67

High (45.4)^2a

Ovomucoidⁱ

P01003;15709 (IOVO;IOVO)

0.11

0.05

Intermediate (2.2)^2a

UDP-glucuronic acid decarboxylase 1

11592 (G1NPP3;E1BV28)

0.10

0.07

Intermediate (2.2)^2a

Uncharacterized/similar to mucin

5306;6950;1527 (G1N931;E1C037)

0.10

0.15

Low (1.4)^2a

Insulin-like growth factor-binding protein 7 (IGFBP7)

18344 (G1N8M9;F1NVP4)

0.10

0.07

Intermediate (3.6)^2a

Ganglioside GM2 activator^{a g, t}

24235 (G1N3B0;Q5ZK02)

0.10

-

High (12.9)^2a

Synthenin-1 (SDCBP)^t

24420 (G1NEY6;Q5ZHM8)

0.10

-

Intermediate (2.8)^2a

Similar to procollagen C-endopeptidase enhancer

30796 (G1N443;F1NH70)

0.10

-

-