Fig. 2

The fatty acid composition of HepG2-SF TG after fatty acid supplementation. HepG2-SF TG composition was analyzed by gas liquid chromatography after 7 days incubation in fatty acid supplemented medium (100 μM). Panel a and b are high and low abundant fatty acids present in the TG, respectively. Content of individual fatty acids (x-axis), analyzed as extracted FAME, are averaged over three biological replicas and displayed as % of total TG derived FAME measured (y-axis). The supplemented TFAs esterify at different levels into HepG2-SF TG and EA is found at approximately three times the level of transVA. The analysis shows that the supplemented fatty acids are taken up and metabolized by HepG2-SF cells. In addition, it demonstrates that EA is accumulating in TG when compared to the other experimental groups. *significantly different from Control at p < 0.05. Fatty acids significantly differing between experimental groups at p < 0.05 are marked with: a) different from OA, b) different from cisVA and c) different from transVA