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Table 2 Differentially regulated secreted proteins revealed by DIGE and identified by mass spectrometry

From: Hepatocytes respond differently to major dietary trans fatty acid isomers, elaidic acid and trans-vaccenic acid

   

EA/transVA

transVA/cisVA

Protein name

Uniprot name

spot no

Av. Ratio

p-value

Av. Ratio

p-value

14-3-3 protein zeta/delta

1433Z/P63104

2180

  

−1.43

3.0E-02

Aldose 1-epimerase

GALM/Q96C23

1832

2

2.5E-03

  

Alpha-1-antitrypsin

A1AT/P01009

1330

1.79

3.6E-04

  

Alpha-1-antitrypsin

 

1342

1.96

3.9E-03

  

Alpha-2-HS-glycoprotein

FETUA/P02765

1400

−1.51

3.4E-02

  

Apolipoprotein A1

APOA1/P02647

2333

1.57

2.0E-04

  

Apolipoprotein A4

APOA4/P06727

1737

1.57

7.3E-03

1.39

4.8E-02

Apolipoprotein A4

 

1741

1.7

1.7E-04

1.4

2.8E-03

Apolipoprotein A4

 

1743

1.43

2.3E-02

  

Apolipoprotein E

APOE/P02649

2030

1.39

5.9E-03

  

Carboxypeptidase E

CBPE/P16870

1417

−1.78

1.3E-03

  

Chloride intracellular channel protein1

CLIC1/O00299

2106

1.75

1.9E-03

  

Complement C3

CO3/P01024

1675

−1.41

3.1E-02

  

Complement C3

 

1706

−1.51

4.6E-03

  

Complement C3

 

1708

−1.51

4.6E-03

  

Complement C3

 

1710

−1.51

8.8E-04

  

Complement factor B

CFAB/P00751

726

−1.32

2.5E-03

  

Endoplasmin

ENPL/P14625

737

−1.51

4.8E-02

  

Proteasome subunit alpha type-5

PSA5/P28066

2203

  

−1.37

4.7E-02

Protein AMBP

AMBP/P02760

2042

1.62

2.2E-03

  

Serotransferrin

TRFE/P02787

1005

1.56

8.0E-04

  

Serotransferrin

 

1007

2.02

2.7E-04

  

Serotransferrin

 

1033

1.65

1.5E-04

  

Serotransferrin

 

1765

1.43

1.1E-02

  
  1. The secretome of hepatocytes supplemented with transVA, EA, or cisVA were analyzed by DIGE and subsequently the regulated proteins were identified by LC-MS/MS analyses. In the table proteins are reported with protein name, Uniprot name/accession number, 2D gel spot number (spot no), Average ratio (Av.ratio), and p-value for individual comparisons