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Table 2 Differentially regulated secreted proteins revealed by DIGE and identified by mass spectrometry

From: Hepatocytes respond differently to major dietary trans fatty acid isomers, elaidic acid and trans-vaccenic acid

    EA/transVA transVA/cisVA
Protein name Uniprot name spot no Av. Ratio p-value Av. Ratio p-value
14-3-3 protein zeta/delta 1433Z/P63104 2180    −1.43 3.0E-02
Aldose 1-epimerase GALM/Q96C23 1832 2 2.5E-03   
Alpha-1-antitrypsin A1AT/P01009 1330 1.79 3.6E-04   
Alpha-1-antitrypsin   1342 1.96 3.9E-03   
Alpha-2-HS-glycoprotein FETUA/P02765 1400 −1.51 3.4E-02   
Apolipoprotein A1 APOA1/P02647 2333 1.57 2.0E-04   
Apolipoprotein A4 APOA4/P06727 1737 1.57 7.3E-03 1.39 4.8E-02
Apolipoprotein A4   1741 1.7 1.7E-04 1.4 2.8E-03
Apolipoprotein A4   1743 1.43 2.3E-02   
Apolipoprotein E APOE/P02649 2030 1.39 5.9E-03   
Carboxypeptidase E CBPE/P16870 1417 −1.78 1.3E-03   
Chloride intracellular channel protein1 CLIC1/O00299 2106 1.75 1.9E-03   
Complement C3 CO3/P01024 1675 −1.41 3.1E-02   
Complement C3   1706 −1.51 4.6E-03   
Complement C3   1708 −1.51 4.6E-03   
Complement C3   1710 −1.51 8.8E-04   
Complement factor B CFAB/P00751 726 −1.32 2.5E-03   
Endoplasmin ENPL/P14625 737 −1.51 4.8E-02   
Proteasome subunit alpha type-5 PSA5/P28066 2203    −1.37 4.7E-02
Protein AMBP AMBP/P02760 2042 1.62 2.2E-03   
Serotransferrin TRFE/P02787 1005 1.56 8.0E-04   
Serotransferrin   1007 2.02 2.7E-04   
Serotransferrin   1033 1.65 1.5E-04   
Serotransferrin   1765 1.43 1.1E-02   
  1. The secretome of hepatocytes supplemented with transVA, EA, or cisVA were analyzed by DIGE and subsequently the regulated proteins were identified by LC-MS/MS analyses. In the table proteins are reported with protein name, Uniprot name/accession number, 2D gel spot number (spot no), Average ratio (Av.ratio), and p-value for individual comparisons