Fig. 1

Biochemical parameters and validation of detergent resistant membrane microdomains. Isolated DRM microdomains showing that the 4^th and 5^th fractions were enriched in cholesterol (a), protein (c) and presenting high ACE activity (b). Representative silver stained 1D SDS-PAGE and Western Blot images of fractions 4 through 6 (d). Immunoblot experiments were performed for caveolin-1 and polymerase I and transcript release factor (PTRF) as DRM microdomain protein markers. Fractions 4 and 5 were chosen for further proteomic experiments as DRM microdomains enriched fractions. Molecular weight protein standards (M) are also shown