Fig. 1

2D-DIGE analysis of secreted proteins from Nipponbare suspension-cultured cells 0 h and 24 h after inoculation with Xoo. Secreted protein (150 μg) from each sample were labeled with Cy3 and Cy5 respectively, loaded and separated on 24 cm strips (pH = 4–7) in the first dimension, followed by electrophoresis on 12.5 % SDS-PAGE gels in the second dimension. Gels were scanned with a DIGE scanner, merged and shown as a grayscale image. Protein spots responsive to Xoo-inoculation are marked by numbered arrows and identified in Tables 1 and 2. Relative mobility (Mr) markers were the protein ladder kit purchased from GE Healthcare (USA)