Fig. 4
From: Secretome analysis of rice suspension-cultured cells infected by Xanthomonas oryzae pv.oryza (Xoo)
Subcellular location of three identified Xoo secreted proteins in rice protoplast cells. To exclude interference of the GFP protein with the location of the tested genes, bacterial blight genes were cloned into the pGW505 (_505, 35S: Gene-GFP fusion) and pGW506 (_506, 35S: GFP- Gene fusion) vectors. GFP signals were detected 16 h after transient expression in rice protoplasts. Images of rice protoplasts observed under bright light, GFP, chlorophyll channels, and false-colour signal images are shown in panels from left to right, respectively. Superimposed images are shown in the rightmost panel. GFP protein fusions of Xoo3479, Xoo3654 and Xoo0842 were all observed on plasma membranes. Bar = 10 μm