Fig. 5

Over-expression of Xoo_3654 (X2) in PXO124 and its effects on pathogenicity. a: Growth curve of construct strains pHMX2- PXO124 and pHM1-PXO124. Compared with mock plasmid pHM1, X2 slightly reduced the growth rate. b: Expression of X2 gene in construct strains pHMX2- PXO124 and pHM1-PXO124. Total RNA was extracted from pHM1-PXO124 and pHMX2-PXO124, and 500 ng RNA was reverse transcribed by the iScript cDNA synthesis kit (Bio-RAD) and quantified using qRT-PCR. 16 s-rDNA was used to normalize total RNA amounts. Expression levels of X2 in pHMX2-PXO124 are more than fifteen times higher than in pHM1-PXO124. c: Pathogenicity test showing reduction of lesion length in young leaves inoculated with pHMX2- PXO124 compared to those inoculated with pHM1-PXO124. d: Phenotypes of lesions in 21-dyas rice leaves inoculated with pHMX2- PXO124 and pHM1-PXO124 for 14 days