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Fig. 1 | Proteome Science

Fig. 1

From: Stathmin involvement in the maternal embryonic leucine zipper kinase pathway in glioblastoma

Fig. 1

Proteomics analysis of differentially expressed proteins in U87MG transfected with siRNA for MELK compared with U87MG transfected with non-target control. a Representative two-dimensional electrophoresis gels (2DE). Protein extracts (200 μg) were applied to 2DE using IPG pH 3-10NL (7 cm) for isoelectric focusing and 12.5 % SDS-PAGE mini gels as a second dimension. Gel image analysis was performed using ImageMaster 2D Platinum v.7.0 software. The arrows indicate selected protein spots from mass spectrometry analysis. b Proteins differentially expressed (up-regulated and downregulated) in the glioma cell line U87MG transfected with siRNA for MELK compared with U87MG cells transfected with non-target control (NTC). The expression levels of 12 differentially expressed protein spots were quantified on the basis of the normalised volume of the 2DE spots (% vol) for each group. These data were analysed via ANOVA (p < 0.05). The data are reported as the mean ± SD. One of the proteins, Stathmin (STMN1), exhibited lower expression levels when MELK was silenced

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