Fig. 2
From: Stathmin involvement in the maternal embryonic leucine zipper kinase pathway in glioblastoma
Gene expression levels in astrocytomas of different malignant grades (AGI, AGII, AGIII and GBM) and non-neoplastic brain tissues (NN). Expression levels of MELK a and STMN1 (b) were analyzed by quantitative real-time PCR (qRT-PCR) using the SYBR Green method. The following equation was applied to calculate gene expression levels: 2-∆Ct, in which ∆Ct = Ct of the target gene-geometric mean Ct of reference genes. Horizontal bars indicate the median relative expression levels of all tissues. The differences in MELK and STMN1 expression levels among the groups were statistically significant (Kruskal-Wallis test, p < 0.0001). MELK expression levels were higher in GBM samples (median value = 0.28) than the other groups. STMN1 median expression levels for NN, AGI, AGII, AGIII and AGIV were 21.7, 5.7, 16.9, 21.8 and 12.2, respectively. A post-hoc Dunn’s test was used to calculate the differences in expression between NN and each astrocytoma group (**** p < 0.00005, *** p < 0.0005, ** p < 0.005 and * p < 0.05). The results are presented in log10 scale for better visualisation