Table 1 Protein identification by MALDI-TOF-TOF MS of tryptic peptides obtained from 2DE spots of siRNA-NTC- and siRNA-MELK-transfected cells
From: Stathmin involvement in the maternal embryonic leucine zipper kinase pathway in glioblastoma
Protein spot Id | Acc UniProt | Protein identification | Protein fold change MELK/NTC (% Spot Vol) | Protein score | MW/pI | Protein matches | Coverage (%) |
|---|---|---|---|---|---|---|---|
1 | ANXA2_HUMAN | Annexin A2 | 3.49 ± 0.08 | 1003 | 38832/7.57 | 13 | 41.6 |
2 | TCPG_HUMAN | T-complex protein 1 subunit gamma | 0.55 ± 0.06 | 395 | 61104/6.1 | 9 | 19.4 |
3 | PSME2_HUMAN | Proteasome activator complex subunit 2 | 3.24 ± 0.13 | 356 | 27532/5.44 | 5 | 28.5 |
4 | STIP1_HUMAN | Stress-induced-phosphoprotein 1 | 0.52 ± 0.02 | 325 | 63266/6.4 | 6 | 13.8 |
5 | ENOG_HUMAN | Gamma-enolase | 0.51 ± 0.09 | 246 | 47610/4.91 | 4 | 11.1 |
6 | ALDR_HUMAN | Aldose reductase | 0.50 ± 0.04 | 101 | 36252/6.51 | 3 | 11.4 |
7 | ATP5H_HUMAN | ATP synthase subunit d, mitochondrial | 0.44 ± 0.02 | 152 | 18548/5.21 | 4 | 24.2 |
8 | STMN1_HUMAN | Stathmin | 0.52 ± 0.01 | 145 | 17302/5.76 | 2 | 16.8 |
9 | C1QBP_HUMAN | Complement component 1 Q subcomponent-binding protein, mitochondrial | 1.86 ± 0.06 | 141 | 31761/4.74 | 2 | 9.9 |
10 | TPP1_HUMAN | Tripeptidyl-peptidase 1 | 2.21 ± 0.04 | 139 | 61761/6.01 | 4 | 10.7 |
11 | SODC_HUMAN | Superoxide dismutase [Cu-Zn] | 0.42 ± 0.06 | 104 | 16164/5.7 | 1 | 9.1 |
12 | LMNA_HUMAN | Lamin-A/C | 2.08 ± 0.09 | 83 | 74424/6.57 | 4 | 6 |