Fig. 2

CD13 expression is enhanced on THP-1 derived DCs and human DCs as stimulated with HKTB. a Western blotting showing the changes in CD13 level in THP-1 derived DCs treated with LPS and TNF-alpha and HKTB with respect to medium-treated control cells. Protein expressions were quantified by densitometric analysis and CD13 were normalized to the beta-actin of each sample. These experiments were each conducted three times and the results are shown. b Flow cytometry analysis of THP-1 derived DCs, and human DCs stained for CD13 antibody and appropriate isotype controls (open histograms). The filled black and gray histograms represent CD13 expression of cells with or without HKTB treatment, respectively. The fluorescence intensity of CD13 was shown as means ± SEM (n = 3)