Fig. 4

CD13 is involved in the inhibition of CD1 expression and T cell response by HKTB. a CD1 expression on dendritic cells infected with or without HKTB for 48 h was measured by flow cytometry. The fluorescence intensity of CD1a was expressed relative to isotype antibody (100 %) and shown as means ± SEM (n = 5). The fluorescence intensity of CD1b expression (b) and CD1c expression (c) were shown as means ± SEM (n = 4). d CFSE-labeled T cells were cultured with dendritic cells for six days and T cell proliferation was analyzed by flow cytometry. These experiments were conducted with four times and representative result of proliferation index was shown. * P <0.05. e CD1 expression on dendritic cells infected with or without LAM for 48 h was measured by flow cytometry. The fluorescence intensity of CD1a was expressed relative to isotype antibody (100 %) and shown as means ± SEM (n = 5). The fluorescence intensity of CD1b expression (f) and CD1c expression (g) were shown as means ± SEM (n = 4). h The results of T cell proliferation were representative of four different experiments