Fig. 2From: Investigation of proteome changes in osteoclastogenesis in low serum culture system using quantitative proteomicsValidation of the LC-MS/MS data and osteoclast formation by western blot analysis. Characteristic biomarkers of osteoclasts were seen. TRAP, CTSK, and MMP-9 were significantly upregulated in osteoclasts. Similar to the results from LC-MS/MS, ANXA1 and histone H4 were upregulated in osteoclasts compared to RAW 264.7 cellsBack to article page