Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers

Table 2 Primers used for the quantitative real-time RT-PCR analysis

Gene name	Sequence (5’–3’)
β-actin	Sense: TCTTGGGTATGGAGTCCTG Antisense: TAGAAGCATTTGCGGTGG
FGA	Sense: GCAGAACAGCATCCAGGAGCAGG Antisense: TCCACCTGGTAATCAAAACTTCTAGCAC
CA2	Sense: CACTGGCACGAGCACTTC Antisense: ACTTCACGCCATCCACAGT
KRT7	Sense: CTGGACGGGTTGTTAAAT Antisense: TCCGCTTCGTAGAGAGAT
GRTP1	Sense: GGCTGCTCCAACGCCCACTT Antisense: CGCAACGCCTTCTGCTCTTT
MnSOD	Sense: CTGACCTGCCCTACGACT Antisense: TGGTATGATTGATATGACCC
LOC429524	Sense: GGAGGAAATGCGGCGCTTAG Antisense: GGCTGGACGAGACGCTGTTGA
ATP5A1	Sense: CAGTTTGGGTCTGATTTGG Antisense: AGCTTAGCTTCCGTCTGG
FKBP4	Sense: TACCTCCCAATGCTACGC Antisense: CCTTCGCCTTTCTTACGG
GOT1	Sense: GCACAGACCCTACTCCAGAC Antisense: AAGCCCTCGGAGACAAAG
LMNA	Sense: GGGGAACTGGCAGGTGAAGC Antisense: CCTCGTCGTCGTCGTTGATG
PTGDS	Sense: CCGAGGTCTTTTGTTTG Antisense: AGGAGGGGGACTTTGATG
HSPβ1	Sense: CAAACACGAGGAGAAACA Antisense: CGTTTATTCAAGGCACTG
Apo A-I	Sense: TCCGCTTCGTAGAGAGATGTG Antisense: TCAGCGTGTCCAGGTTGTC

β-actin acts as internal control; FGA encodes the fibrinogen alpha chain, CA2 encodes carbonic anhydrase II, Otokeratin encodes the cytokeratin otokeratin protein, GRTP1 was predicted to encode the growth hormone-regulated TBC protein 1 protein, MnSOD encodes the MnSOD protein, LOC429524 was predicted to encode a transcription factor 24-like protein, ATP5A1 encodes the ATP synthase subunit alpha protein, FKBP4 encodes the PPIase FKBP4 protein, GOT1 encodes the aspartate aminotransferase 1 protein, LMNA encodes the lamin-A protein, PTGDS encodes the prostaglandin-H2 D-isomerase precursor protein, HSPβ1 encodes the HSPβ1 protein, Apo A-I encodes the Apo A-I protein

ISSN: 1477-5956