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Table 3 MS/MS spectral features of unadducted and HNE-adducted goat (Capra hircus) meat myoglobin peptides

From: Proteomic based approach for characterizing 4-hydroxy-2-nonenal induced oxidation of buffalo (Bubalus bubalis) and goat (Capra hircus) meat myoglobins

Peptide positiona)

Peptide sequenceb)

Modification and mass shift

Precursor m/z

b and y ions identifiedc)

17–31

VEADVAGHGQEVLIR

Unadducted

1592.77

b ions: 100.08 (b1), 229.12 (b2), 300.16 (b3), 415.16 (b4), 514.25 (b5), 585.29 (b6), 642.31 (b7), 779.37 (b8), 836.39 (b9), 964.45 (b10), 1093.49 (b11), 1192.56 (b12), 1305.64 (b13), 1418.73 (b14)

y ions: 174.11 (y1), 287.20 (y2), 400.28 (y3), 499.35 (y4), 628.39 (y5), 756.45 (y6), 813.47 (y7), 950.53 (y8), 1107.55 (y9), 1078.59 (y10), 1177.66 (y11), 1292.68 (y12), 1363.72 (y13), 1492.76 (y14)

17–31

VEADVAGHGQEVLIR

HNE + 158.1

1751.01

b ions: 100.08 (b1), 229.12 (b2), 300.16 (b3), 415.16 (b4), 514.25 (b5), 585.29 (b6), 642.31 (b7), 937.50 (b8), 994.52 (b9), 1122.58 (b10), 1251.62 (b11), 1350.69 (b12), 1463.77 (b13), 1576.86 (b14)

y ions: 174.11 (y1), 287.20 (y2), 400.28 (y3), 499.35 (y4), 628.39 (y5), 756.45 (y6), 813.47 (y7), 1108.66 (y8), 1165.68 (y9), 1236.72 (y10), 1335.79 (y11), 1450.81 (y12), 1521.85 (y13), 1650.89 (y14)

32–42

LFTGHPETLEK

Unadducted

1271.43

b ions: 114.09 (b1), 261.16 (b2), 362.21 (b3), 419.23 (b4), 556.29 (b5), 653.34 (b6), 782.38 (b7), 883.43 (b8), 996.52 (b9), 1125.56 (b10)

y ions: 146.11 (y1), 275.15 (y2), 388.23 (y3), 489.28 (y4), 618.32 (y5), 715.38 (y6), 852.43 (y7), 909.46 (y8), 1010.50 (y9), 1157.57 (y10)

32–42

LFTGHPETLEK

HNE + 158.1

1429.67

b ions: 114.09 (b1), 261.16 (b2), 362.21 (b3), 419.23 (b4), 714.42 (b5), 811.47 (b6), 940.51 (b7), 1041.56 (b8), 1154.65 (b9), 1283.69 (b10)

y ions: 146.11 (y1), 275.15 (y2), 388.23 (y3), 489.28 (y4), 618.32 (y5), 715.38 (y6), 1010.56 (y7), 1067.59 (y8), 1168.63 (y9), 1315.70 (y10)

63–77

KHGNTVLTALGGILK

Unadducted

1521.82

b ions: 129.10 (b1), 266.16 (b2), 323.18 (b3), 437.23 (b4), 538.27 (b5), 637.34 (b6), 750.43 (b7), 851.47 (b8), 922.51 (b9), 1035.60 (b10), 1092.62 (b11), 1149.64 (b12), 1262.72 (b13), 1375.81 (b14)

y ions: 146.11 (y1), 259.19 (y2), 372.27 (y3), 429.30 (y4), 486.32 (y5), 599.40 (y6), 670.44 (y7), 771.49 (y8), 884.57 (y9), 983.64 (y10), 1084.69 (y11), 1198.73 (y12), 1255.75 (y13), 1392.81 (y14)

63–77

KHGNTVLTALGGILK

HNE + 158.1

1680.06

b ions: 129.10 (b1), 424.29 (b2), 481.31 (b3), 595.36 (b4), 696.40 (b5), 795.47 (b6), 908.56 (b7), 1009.60 (b8), 1080.64 (b9), 1193.73 (b10), 1250.75 (b11), 1307.77 (b12), 1420.85 (b13), 1533.94 (b14)

y ions: 146.11 (y1), 259.11 (y2), 372.27 (y3), 429.30 (y4), 486.32 (y5), 599.40 (y6), 670.44 (y7), 771.49 (y8), 884.57 (y9), 983.64 (y10), 1084.69 (y11), 1198.73 (y12), 1255.75 (y13), 1550.94 (y14)

80–87

GHHEAEVK

Unadducted

905.96

b ions: 58.03 (b1), 195.09 (b2), 332.15 (b3), 461.19 (b4), 532.23 (b5), 661.27 (b6), 760.34 (b7)

y ions: 146.11 (y1), 245.17 (y2), 374.22 (y3), 445.25 (y4), 574.30 (y5), 711.36 (y6), 848.41 (y7)

80–87

GHHEAEVK

HNE + 158.1

1064.20

b ions: 58.03 (b1), 353.22 (b2), 490.28 (b3), 619.32 (b4), 690.36 (b5), 819.40 (b6), 918.47 (b7)

y ions: 146.11 (y1), 245.17 (y2), 374.22 (y3), 445.25 (y4), 574.30 (y5), 711.36 (y6), 1006.54 (y7)

88–96

HLAESHANK

Unadducted

1271.40

b ions: 138.07 (b1), 251.15 (b2), 322.19 (b3), 451.23 (b4), 538.26 (b5), 675.32 (b6), 746.36 (b7), 860.40 (b8), 988.50 (b9), 1125.56 (b10)

y ions: 146.11 (y1), 283.16 (y2), 411.26 (y3), 525.30 (y4), 596.34 (y5), 733.40 (y6), 820.43 (y7), 949.47 (y8), 1020.51 (y9), 1133.59 (y10)

88–96

HLAESHANK

HNE + 158.1

1164.32

b ions: 296.20 (b1), 409.28 (b2), 480.38 (b3), 609.36 (b4), 696.39 (b5), 833.45 (b6), 904.49 (b7), 1018.53 (b8)

y ions: 146.11 (y1), 260.15 (y2), 331.19 (y3), 468.24 (y4), 555.28 (y5), 684.32 (y6), 755.36 (y7), 868.44 (y8)

88–98

HLAESHANKHK

Unadducted

1271.40

b ions: 138.07 (b1), 251.15 (b2), 322.19 (b3), 451.23 (b4), 538.26 (b5), 675.32 (b6), 746.36 (b7), 860.40 (b8), 988.50 (b9), 1125.56 (b10)

y ions: 146.11 (y1), 283.16 (y2), 411.26 (y3), 525.30 (y4), 596.34 (y5), 733.40 (y6), 820.43 (y7), 949.47 (y8), 1020.51 (y9), 1133.59 (y10)

88–98

HLAESHANKHK

HNE + 158.1

1429.64

b ions:138.07 (b1), 251.15 (b2), 322.19 (b3), 451.23 (b4), 538.26 (b5), 675.32 (b6), 746.36 (b7), 860.40 (b8), 988.50 (b9), 1283.69 (b10)

y ions: 146.11 (y1), 441.30 (y2), 569.39 (y3), 683.43 (y4), 754.47 (y5), 891.53 (y6), 978.56 (y7), 1107.60 (y8), 1178.64 (y9), 1291.72 (y10)

103–118

YLEFISDAIIHVLHAK

Unadducted

1869.19

b ions: 164.07 (b1), 277.16 (b2), 406.20 (b3), 553.27 (b4), 666.35 (b5), 753.38 (b6), 868.41 (b7), 939.45 (b8), 1052.53 (b9), 1165.61 (b10), 1302.67 (b11), 1401.74 (b12), 1514.83 (b13), 1651.88 (b14), 1722.92 (b15)

y ions: 146.11 (y1), 217.14 (y2), 354.20 (y3), 467.29 (y4), 566.35 (y5), 703.41 (y6), 816.50 (y7), 929.58 (y8), 1000.62 (y9), 1115.65 (y10), 1202.68 (y11), 1315.76 (y12), 1462.83 (y13), 1591.95 (y14), 1704.96 (y15)

103–118

YLEFISDAIIHVLHAK

HNE + 158.1

2027.43

b ions: 164.07 (b1), 277.16 (b2), 406.20 (b3), 553.27 (b4), 666.35 (b5), 753.38 (b6), 868.41 (b7), 939.45 (b8), 1052.53 (b9), 1165.61 (b10), 1460.80 (b11), 1559.87 (b12), 1672.96 (b13), 1810.02 (b14), 1881.05 (b15)

y ions: 146.11 (y1), 217.14 (y2), 354.20 (y3), 467.29 (y4), 566.35 (y5), 861.54 (y6), 974.63 (y7), 1087.71 (y8), 1158.75 (y9), 1273.78 (y10), 1360.81 (y11), 1473.89 (y12), 1620.96 (y13), 1750.00 (y14), 1863.09 (y15)

103–118

YLEFISDAIIHVLHAK

Unadducted

1869.19

b ions: 164.07 (b1), 277.16 (b2), 406.20 (b3), 553.27 (b4), 666.35 (b5), 753.38 (b6), 868.41 (b7), 939.45 (b8), 1052.53 (b9), 1165.61 (b10), 1302.67 (b11), 1401.74 (b12), 1514.83 (b13), 1651.88 (b14), 1722.92 (b15)

y ions: 146.11 (y1), 217.14 (y2), 354.20 (y3), 467.29 (y4), 566.35 (y5), 703.41 (y6), 816.50 (y7), 929.58 (y8), 1000.62 (y9), 1115.65 (y10), 1202.68 (y11), 1315.76 (y12), 1462.83 (y13), 1591.95 (y14), 1704.96 (y15)

103–118

YLEFISDAIIHVLHAK

HNE + 158.1

2027.43

b ions: 164.07 (b1), 277.16 (b2), 406.20 (b3), 553.27 (b4), 666.35 (b5), 753.38 (b6), 868.41 (b7), 939.45 (b8), 1052.53 (b9), 1165.61 (b10), 1302.67 (b11), 1401.74 (b12), 1514.83 (b13), 1810.02 (b14), 1881.05 (b15)

y ions: 146.11 (y1), 217.14 (y2), 512.33 (y3), 625.42 (y4), 724.48 (y5), 861.54 (y6), 974.63 (y7), 1087.71 (y8), 1158.75 (y9), 1273.78 (y10), 1360.81 (y11), 1473.89 (y12), 1620.96 (y13), 1750.00 (y14), 1863.09 (y15)

119–133

HPSDFGADAQGAMSK

Unadducted

1518.62

b ions: 138.07 (b1), 235.12 (b2), 322.15 (b3), 437.18 (b4), 584.25 (b5), 641.27 (b6), 712.31 (b7), 827.33 (b8), 898.37 (b9), 1026.43 (b10), 1083.45 (b11), 1154.49 (b12), 1285.53 (b13), 1372.56 (b14)

y ions: 146.11 (y1), 233.14 (y2), 364.18 (y3), 435.22 (y4), 492.4 (y5), 620.30 (y6), 691.33 (y7), 806.36 (y8), 877.40 (y9), 934.42 (y10), 1081.49 (y11), 1196.51 (y12), 1283.55 (y13), 1380.60 (y14)

119–133

HPSDFGADAQGAMSK

HNE + 158.1

1676.86

b ions: 296.20 (b1), 393.25 (b2), 480.28 (b3), 595.67 (b4), 742.85 (b5), 799.40 (b6), 870.44 (b7), 985.46 (b8), 1056.50 (b9), 1184.56 (b10), 1241.58 (b11), 1312.62 (b12), 1443.66 (b13), 1530.69 (b14)

y ions: 146.11 (y1), 233.14 (y2), 364.18 (y3), 435.22 (y4), 492.24 (y5), 620.30 (y6), 691.33 (y7), 806.36 (y8), 877.40 (y9), 934.42 (y10), 1081.49 (y11), 1196.51 (y12), 1283.55 (y13), 1380.60 (y14)

  1. a) Amino acid position in the goat Mb; b) Amino acid sequence in the goat Mb; c) Observed signals assigned as b or y ions. Ions showing shift in the mass by 156 Da indicating HNE adduction with respect to corresponding ions in unmodified peptides are highlighted in bold fonts
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